Comparative transcriptome analysis between 'Meiguicheng' (Citrus sinensis) and its seedless mutant during early sexual organs development
文献类型： 外文期刊
作者： Wen, Shouxing 1 ; Lin, Xiongjie 1 ; He, Jingyi 2 ; Zhang, Ling-Yuan 3 ; Wang, Xianda 1 ; Zhang, Lijie 1 ; Lin, Yanjin 1 ; Hu, Hanqing 1 ; Xu, Jiahui 1 ; Huang, Jing-Hao 1 ;
作者机构： 1.Fujian Acad Agr Sci, Fruit Res Inst, Fujian Key Lab Plant Nutr & Fertilizer, Fuzhou 350013, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350000, Peoples R China
3.Fujian Univ Tradit Chinese Med, Fuzhou 350122, Peoples R China
关键词： Citrus; Female sterility; Seedlessness; Transcription factor
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN： 0304-4238
年卷期： 2024 年 338 卷
页码：
收录情况： SCI
摘要： Female sterility is one of the keys determining seedlessness formation in citrus. However, the molecular basis for female sterility in citrus is still far from fully understood. In the present study, differentially expressed genes were identified in sexual organs of 'Meiguicheng' orange (Citrus sinensis) and its seedless mutant, candidate genes were then verified by RT-PCR, live-cell imaging technique and physiological biochemistry. Using high-throughput sequencing, 96 and 710 differentially expressed genes (DEGs) were captured in the ovaries and anthers, respectively, during megasporogenesis and microsporogenesis (Ph1). The number of DEGs in ovaries and anthers increased to 325 and 1760, respectively, post gametophyte abortion (Ph2). GO analysis and non-structural carbohydrates determination displayed that disturbances to starch and sucrose metabolism in anthers were responsible for male sterility. In Ph1 ovaries, five DEGs were enriched in reproduction process, one of which (Cs7g06410) was also involved in signaling. Whereas in Ph2 ovaries, eight DEGs were enriched in reproductive process, accompanied with four other DEGs involved in plant hormone signal transduction. PlantTFDB prediction indicated that, in the ovary, four transcription factors (TFs) at Ph1 were differentially expressed. Our findings displayed that Cs2g16620 (PIN1), Cs7g06410 (NPH3), and Cs3g23070 (MYB) might play pivotal roles in citrus female sterility through manipulating auxin transport and accumulation within the ovules, providing potential genes for further investigations on female gametophyte development and transgenic breeding in citrus.
- 相关文献
作者其他论文 更多>>
-
Functional Characterization of KNOX and BELL Genes in Temperature-Responsive Floral Morphogenesis of Passion Fruit (Passiflora edulis)
作者：Jiang, Xinni;Miao, Jie;Zu, Weifan;Zhou, Ruohan;Zheng, Lexin;Wei, Ying;Lai, Chunmei;Zheng, Ping;Qin, Yuan;Niu, Xiaoping;Qin, Rongjuan;Zheng, Ping;Qin, Yuan;Wei, Xiuqing;Xu, Jiahui
关键词：
TALE gene; evolutionary analysis; floral organogenesis; heat/cold stress -
Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit
作者：Yang, Hanbing;Wang, Lifeng;Zheng, Ping;Li, Junzhang;Zou, Yutong;Wang, Lulu;Feng, Xinyuan;Qin, Yuan;Zhuang, Yuhui;Wei, Xiuqing;Xu, Jiahui
关键词：
Passiflora edulis ;VLN gene; actin filament; reproduction development -
Phosphoproteomic Profiling Deciphers Heat-Stress-Responsive Mechanisms in Passion Fruit
作者：Li, Liang;Tang, Yajun;Yu, Dong;Zhou, Ping;Wei, Xiuqing;Xu, Jiahui;Liu, Zhicheng
关键词：passion fruit; heat stress; floral buds; phosphorylation
-
The accumulation pattern of sugars and organic acids in diverse fruit pulps coordinates sweet-sour taste of passion fruit
作者：Tang, Yajun;Chen, Jingyuan;Li, Liang;Tang, Limei;Huang, Weijie;Wei, Xiuqing;Xu, Jiahui;Wang, Pengjie
关键词：Passion fruit; Fruit pulp; Pulp neck; Fruit flavor; Sugar; Organic acid
-
Effective Identification of Variety and Origin of Chenpi Using Hyperspectral Imaging Assisted with Chemometric Models
作者：Liu, Hangxiu;Wang, Youyou;Wang, Yiheng;Wang, Jingyi;Zhong, Xinyi;Yang, Jian;Wang, Yiheng;Yuan, Qingjun;Wang, Yiheng;Yuan, Qingjun;Wang, Jingyi;Yuan, Qingjun;Wang, Jingyi;Yang, Jian;Hu, Hanqing
关键词：Chenpi; variety and origin; rapid identification; hyperspectral imaging; machine learning
-
Genetic diversity and comprehensive evaluation of physicochemical traits in Phyllanthus emblica L. for breeding and resource utilization
作者：Wang, Jianchao;Ali, Muhammad Moaaz;Wu, Yongjie;Zhang, Ge;Chen, Jianqing;Chen, Faxing;Wang, Jianchao;Zhang, Xiaoyan;Xie, Lixue;Zhang, Lijie;Li, Tao
关键词：Amla; Fruit quality; Diversity analysis; Tropical and subtropical fruit; Resource conservation
-
Is the Cultivation of Dictyophora indusiata with Grass-Based Substrates an Efficacious and Sustainable Approach for Enhancing the Understory Soil Environment?
作者：Li, Jing;Jiang, Fengju;Di, Xiaoyue;Lai, Qi;Feng, Dongwei;Zeng, Yi;Lei, Yufang;Yin, Yijia;He, Xiuling;Liu, Penghu;Lin, Zhanxi;Lin, Dongmei;Jiang, Fengju;Di, Xiaoyue;Lai, Qi;Feng, Dongwei;Zeng, Yi;Lin, Biaosheng;Lin, Xiongjie
关键词：bamboo mushroom;
Cenchrus fungigraminus ; soil organic acids; spent mushroom substrates; understory environment
