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Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice

文献类型： 外文期刊

作者： Gong, Pan 1 ; Luo, Yanmin 1 ; Huang, Fudeng 2 ; Chen, Yaodong 1 ; Zhao, Chaoyue 1 ; Wu, Xin 1 ; Li, Kunyu 1 ; Yang, Xi 1 ; C 1 ;

作者机构： 1.Zhejiang Univ, Dept Agron, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Univ, Coll Agr & Biotechnol, Expt Teaching Ctr, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China

4.Zhejiang Acad Agr Sci, Environm Resources & Soil Fertilizer Inst, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Oryza sativa L; OsPLS2; Upf1-like helicase; Premature leaf senescence; Light-dependent; ROS

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2019 年 100 卷 1-2 期

页码：

收录情况： SCI

摘要： Key messageThe OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2.AbstractLeaf senescence is a very complex physiological process controlled by both genetic and environmental factors, however its underlying molecular mechanisms remain elusive. In this study, we report a novel Oryza sativa premature leaf senescence mutant (ospls2). Through map-based cloning, a G-to-A substitution was determined at the 1st nucleotide of the 13th intron in the OsPLS2 gene that encodes a Upf1-like helicase. This mutation prompts aberrant splicing of OsPLS2 messenger and consequent disruption of its full-length protein translation, suggesting a negative role of OsPLS2 in regulating leaf senescence. Wild-type rice accordingly displayed a progressive drop of OsPSL2 protein levels with age-dependent leaf senescence. Shading and light filtration studies showed that the ospls2 phenotype, which was characteristic of photo-oxidative stress and reactive oxygen species (ROS) accumulation, was an effect of irritation by light. When continuously exposed to far-red light, exogenous H2O2 and/or abscisic acid (ABA), the ospls2 mutant sustained hypersensitive leaf senescence. In consistence, light and ROS signal pathways in ospls2 were activated by down-regulation of phytochrome genes, and up-regulation of PHYTOCHROME-INTERACTING FACTORS (PIFs) and WRKY genes, all promoting leaf senescence. Together, these data indicated that OsPLS2 played an essential role in leaf senescence and its disruption triggered light-dependent leaf senescence in rice.

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