A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.
文献类型： 外文期刊
作者： Yu, N-T 1 ; Zhang, Y-L 1 ; Xiong, Z. 3 ; Qu, L. 1 ; Liu, Z-X 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA
4.Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA
关键词： duplex reverse transcription-PCR; nervous necrosis virus; iridovirus; grouper
期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）
ISSN： 0001-723X
年卷期： 2019 年 63 卷 1 期
页码：
收录情况： SCI
摘要： Grouper nervous necrosis virus (GNNV) and grouper iridovirus (GIV) are major grouper-infecting viruses in southern China that can cause serious economic losses. A duplex reverse transcription-PCR (duplex RT-PCR) method was developed for the simultaneous detection of GNNV and GIV. Eight groups of primers specifically targeting the capsid protein genes of GNNV and GIV were designed and analyzed. The primer set GN4 was selected and used to amplify fragments of 887 bp and 319 bp in length from GNNV and GIV, respectively. Furthermore, the duplex PCR assay was shown to be sensitive because it could detect at least 20 pg of plasmid-viral DNA from a mixture of viruses. Using this assay, 18 GNNV infected groupers and 7 GIV infected groupers were detected amongst 41 suspected samples in Hainan. The duplex RT-PCR assay proved to be a rapid, specific, and sensitive method for detecting the two grouper viruses. This method could be used to facilitate better control of fish viruses through early detection.
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