您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Positive correlation between lipid accumulation and gene expression of a copper-containing amine oxidase gene in Chlorella under nitrogen starvation

文献类型： 外文期刊

作者： Jiang, Gu 1 ; Li, Fang 2 ; Wang, Shujun 2 ; Sun, Jinhua 2 ; Liu, Zhiyuan 1 ;

作者机构： 1.Hainan Univ, Ocean Coll, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Haikou 571101, Hainan, Peoples R China

关键词： Chlorella; ChlCAO2; Lipid; Gene expression; CuAOs activity

期刊名称：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS （ 影响因子：4.401； 五年影响因子：5.278 ）

ISSN： 2211-9264

年卷期： 2019 年 40 卷

页码：

收录情况： SCI

摘要： Copper-containing amine oxidases (CuAOs) have a broad range of functions, including respond to abiotic stresses, formation and differentiation of adipocytes in mammals. To verify the correlation between lipid accumulation and CuAOs in microalgae, the full-length gene of one copper amine oxidase isoform 2 from Chlorella sp. HN11 (ChlCAO2) was cloned, and the gene expression and enzymatic activity were determined. Then the correlation between lipid accumulation and CuAOs activities was further examined in C. vulgaris C3 and Chlamydomonas reinhardtii CC425. The results showed that the cDNA of ChlCAO2 was 4135 bp in length with a 2454 bp open reading frame (ORF). No lipid accumulation and up-regulated ChlCAO2 expression were observed in cells cultured in normal medium (the control) within 96 h. The relative fluorescence intensity of lipids in Chlorella cells under N-starvation was 3.03, 4.48, 4.17, and 4.63 times higher than the control at 24 h, 48 h, 72 h, and 96 h, respectively. While the expression of ChlCAO2 significantly increased by 105, 397, 808, and 333 times higher than the control at 24 h, 48 h, 72 h, and 96 h, respectively, the activity of ChlCAOs of N-starved increased correspondingly. When cells under N-starvation were resupplied with N at 48 h (N-recovery), lipid accumulation, the expression of ChlCAO2 and the activity of ChlCAOs decreased significantly (P < 0.05) at 72 h and 96 h compared with N-starvation. Correlation analysis showed that the relative fluorescence intensity of lipid, the expression of ChlCAO2, and the activity of ChlCAOs in Chlorella were markedly positively correlated with each other. Positive correlation between lipid accumulation and CuAO activities were both observed in C. vulgaris C3 and C.reinhardtii CC425.The results indicated that CuAOs play a key role in the process of lipid accumulation induced by nitrogen starvation in green algae.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台