Constitutive expression of a wheat microRNA, TaemiR167a, confers male sterility in transgenic Arabidopsis
文献类型： 外文期刊
作者： Wang, Yukun 3 ; Duan, Wenjing 1 ; Bai, Jianfang 1 ; Wang, Peng 1 ; Yuan, Shaohua 1 ; Zhao, Changping 1 ; Zhang, Liping 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Engn & Tech Res Ctr Hybrid Wheat, 9 Shuguang Pk Middle Rd, Beijing 100097, Peoples R China
2.Municipal Key Lab Mol Genet Hybrid Wheat, Beijing 100097, Peoples R China
3.Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan
关键词： Auxin receptors; microRNA; Wheat; Male sterility; Pollen fertility
期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）
ISSN： 0167-6903
年卷期： 2019 年 88 卷 3 期
页码：
收录情况： SCI
摘要： The miR167 family has been shown to play roles in regulating plant fertility by control the expression of a set of ARF transcription factors. In this study, the precursor of miR167a was cloned from the photo-thermo-sensitive genic male sterile (PTGMS) wheat line BS366. Moreover, we identified the target gene, TaARF8, from PTGMS wheat line BS366, and used the 5-RACE assay to confirm the cleavage site. Overexpression (OE) of TaemiR167a in Arabidopsis induced male sterility phenotypes, and reduced the expression levels of AtARF6 and AtARF8. Meanwhile, OE of TaemiR167a reduced the content of endogenous jasmonic acid (JA) and indole acetic acid (IAA) in transgenic Arabidopsis lines. In PTGMS wheat line BS366, the expression levels of TaemiR167a were up-regulated in anther tissues during pollen development process, while the transcriptional levels of TaARF8 were down-regulated under sterile conditions. Importantly, TaemiR167a was sensitive to low temperature (10 degrees), and was highly expressed in anther tissues of BS366 at pollen developmental stage 3 in sterile environment. Then, we measured the endogenous JA and IAA in BS366 anther tissues, and found that the accumulation of JA and IAA in anther was abnormal during the pollen development process. These results suggested that TaemiR167a-mediated regulation of TaARF8 and changes of endogenous JA and IAA during pollen developmental process took part in the pollen fertility control in PTGMS wheat line BS366.
- 相关文献
作者其他论文 更多>>
-
Transcriptomic and metabolomic analysis provides insights into lignin biosynthesis and accumulation and differences in lodging resistance in hybrid wheat
作者：Yang, Weibing;Zhang, Shengquan;Hou, Qiling;Gao, Jiangang;Wang, Hanxia;Chen, Xianchao;Liao, Xiangzheng;Zhang, Fengting;Zhao, Changping;Qin, Zhilie;Yang, Weibing;Zhang, Shengquan;Zhang, Fengting;Zhao, Changping
关键词：gene expression; lignin synthesis; lodging -resistance; hybrid wheat
-
Comparative transcriptome analysis reveals the impact of daily temperature difference on male sterility in photo-thermo-sensitive male sterile wheat
作者：Niu, Fuqiang;Song, Xiyue;Liu, Zihan;Liu, Yongjie;Bai, Jianfang;Zhang, Tianbao;Yuan, Shaohua;Bai, Xiucheng;Zhao, Changping;Zhang, Fengting;Sun, Hui;Zhang, Liping
关键词：Daily temperature difference; Male sterility; Safe seed production; Transcriptome; Wheat
-
Genome-Wide Identification and Expression Analysis of Sucrose Transporter Gene Family in Wheat Lines under Heat Stress
作者：Hou, Qiling;Gao, Jiangang;Qin, Zhilie;Sun, Hui;Wang, Hanxia;Yuan, Shaohua;Zhang, Fengting;Yang, Weibing
关键词：sucrose transporters; heat stress; gene expression; grain filling; male-sterile lines
-
Characterization and expression analysis of chalcone synthase gene family members suggested their roles in the male sterility of a wheat temperature-sensitive genic male sterile (TGMS) line
作者：Liu, Yongjie;Bai, Jianfang;Yuan, Shaohua;Gao, Shiqing;Liu, Zihan;Li, Yanmei;Zhang, Fengting;Zhao, Changping;Zhang, Liping;Liu, Yongjie;Bai, Jianfang;Yuan, Shaohua;Gao, Shiqing;Liu, Zihan;Li, Yanmei;Zhang, Fengting;Zhao, Changping;Zhang, Liping
关键词：Chalcone synthase; Anther; Pollen exine; Sporopollenin; Phenolics; Temperature-sensitive genic male-sterile; (TGMS)
-
Identification and validation of major-effect quantitative trait locus QMS-5B associated with male sterility in photo-thermo-sensitive genic male sterile wheat
作者：Niu, Fuqiang;Liu, Zihan;Zhang, Fengting;Yuan, Shaohua;Bai, Jianfang;Liu, Yongjie;Li, Yanmei;Zhang, Heng;Zhang, Huishu;Zhao, Changping;Zhang, Liping;Niu, Fuqiang;Song, Xiyue
关键词：
-
Microspore-expressed SCULP1 is required for p-coumaroylation of sporopollenin, exine integrity, and pollen development in wheat
作者：Xu, Lei;Yang, Yanzhi;Wang, Haijun;Du, Jianmei;Bai, Yajun;Li, Lei;Xu, Lei;Du, Jianmei;Bai, Yajun;Li, Lei;Tang, Yimiao;Wang, Dezhou;Su, Shichao;Zhao, Changping
关键词：exine; multicopper oxidase; pollen; sporopollenin; wheat
-
Applications of Xerophytophysiology and Signal Transduction in Plant Production-Flower Qualities in Eustoma grandiflorum Were Improved by Sub-Irrigation
作者：Xu, Hui-Lian;Bai, Jianfang;Bai, Jianfang;Kawabata, Saneyuki;Chang, Tingting
关键词：circadian clock; Eustoma grandiflorum; flower opening; mathematical model; oscillation; osmotic adjustment; PAL (phenylalanine ammonia-lyase); salicylic acid; turgor potential; xerophytophysiology
