文献类型： 外文期刊

作者： Sun, Ai ¹ ; Zhu, Hua ¹ ; Dong, Ying ¹ ; Wang, Wei ¹ ; Hu, Hong Xia ¹ ;

作者机构： 1.Beijing Fisheries Res Inst, Beijing 100068, Peoples R China; Minist Sci & Technol China, Natl Freshwater Fisheries Engn Technol Res Ctr, Lab Biol Technol & Breeding, Beijing Key Lab Fishery Biotechnol, Beijing, Peoples R China

关键词： Acipenser ruthenus; cell line; gene expression; sturgeon; testis; transfection

期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.051； 五年影响因子：2.25 ）

ISSN： 0022-1112

年卷期： 2019 年 94 卷 5 期

页码：

收录情况： SCI

摘要： In this study, a cell line, designated as Acipenser ruthenus testis (ART), was successfully established from testis tissues of the sterlet Acipenser ruthenus and characterized by studying and comparing the expression of specific genes between the cell line and the parent gonad tissues. The results suggested that the developed ART cell line was composed of a mixture of germ cells and somatic cells. Ploidy analysis indicated that the cell line exhibited a high degree of genetic stability and that the cells remained in a good proliferating state after being subcultured to passage 80.