Development of a High-Quality ELISA Method for Dinotefuran Based on a Novel and Newly-Designed Antigen
文献类型： 外文期刊
作者： Zhao, Fangfang 1 ; Liu, Jingkun 5 ; Luo, Jinhui 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Testing Ctr, Haikou 571101, Hainan, Peoples R China
2.Minist Agr, Lab Qual & Safety Risk Assessment Trop Prod Haiko, Haikou 571101, Hainan, Peoples R China
3.Hainan Prov Key Lab Qual & Safety Trop Fruits & V, Haikou 571101, Hainan, Peoples R China
4.Qual Supervis & Inspect Ctr Trop Agroprod, Haikou 571101, Hainan, Peoples R China
5.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Haikou 571101, Hainan, Peoples R China
关键词： dinotefuran; Hapten design; spatial configurations; ELISA; sensitivity and specificity
期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）
ISSN： 1420-3049
年卷期： 2019 年 24 卷 13 期
页码：
收录情况： SCI
摘要： The structure of hapten determines the performance of the antibody and the corresponding detection method. A new type of antigen was designed and synthesized to expose the spatial and characteristic structure of dinotefuran molecule, and a type of high-quality antibody was obtained. The IC50 value of the monoclonal antibody was 5.30 ng/mL and its cross-reactivity (CRs) was less than 2% when reacting with other structurally related analytes. The effects of spatial configurations of hapten on the antibody were visually analyzed while using the appropriate software according to the quality of the antibodies, which showed that the specificity of the antibody is closely related with the exposed structure of hapten. An ELISA assay with an IC50 of 5.66 ng/mL and a linear range of 1.95 to 16.29 ng/mL was developed. The results that were obtained from the ELISA and HPLC methods were equivalent. The results showed that spatial simulation is a crucial method that is used in the designing of hapten to obtain a sensitive and specific antibody. The application of this method will highlight the potential aim and improve the detection efficiency of ELISA.
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