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Expression of the cassava nitrate transporter NRT2.1 enables Arabidopsis low nitrate tolerance

文献类型： 外文期刊

作者： Zou, Liangping 1 ; Qi, Dengfeng 1 ; Sun, Jianbo 1 ; Zheng, Xu 2 ; Peng, Ming 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Henan Agr Univ, Coll Agron, Key Lab Wheat & Maize Crop Sci, Zhengzhou 450046, Henan, Peoples R China

关键词： cassava; MeNRT2; 1 gene; expression pattern; high-affinity nitrate transporter

期刊名称：JOURNAL OF GENETICS （ 影响因子：1.166； 五年影响因子：1.212 ）

ISSN： 0022-1333

年卷期： 2019 年 98 卷 3 期

页码：

收录情况： SCI

摘要： The cassava grows well on low-nutrient soils because of its high-affinity to absorb nitrate. However, the molecular mechanisms by which cassava adapts itself to this environment remain elusive, although we have cloned a putative gene named MeNRT2.1 which has a crucial role in high-affinity nitrate transporter from cassava seeding. Here, the expression pattern of MeNRT2.1 was further assessed using the GUS activity driven by MeNRT2.1 promoter in Arabidopsis transformation plants. The GUS activity was monitored over time following the reduction of nitrate supply. The GUS gene expression not only peaked in roots after 12h in 0.2mM nitrate media, but also stained stems and leaves. Arabidopsis plants with overexpression of MeNRT2.1 increased the biomass compared to the wild type on rich nitrogen (N-full) media. However, chlorate sensitivity analysis showed that Arabidopsis plants expressing MeNRT2.1 were more susceptable to chlorate than wild type. Significantly, after growing for 15 days on media containing 0.2mM nitrate concentration, wild-type plants became yellow or died, while the transgenic MeNRT2.1 Arabidopsis plants maintained normal growth. With significant increases in the amount of 15NO3- uptake in roots, the MeNRT2.1 plants also increased the contents of chlorophyll and nitrate reductase. Taken together, these results demonstrate that MeNRT2.1 has an important role in adaptation to low nitrate concentration as a nitrate transporter.

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