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Functional Identification of Novel Cell Death-inducing Effector Proteins from Magnaporthe oryzae

文献类型： 外文期刊

作者： Guo, Xinrui 2 ; Zhong, Debin 2 ; Xie, Wei 3 ; He, Yanhua 2 ; Zheng, Yueqin 2 ; Lin, Yan 2 ; Chen, Zaijie 2 ; Han, Yijuan; 1 ;

作者机构： 1.Minjiang Univ, Inst Oceanog, Marine Biotechnol Ctr, Fuzhou 350108, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Biotechnol Res Inst, Fuzhou 350003, Fujian, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Plant Protect, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou, Fujian, Peoples R China

4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Magnaporthe oryzae; Rice; Effector; Cell death; Immune response

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2019 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Background Secreted effector proteins play critical roles in plant-fungal interactions. The Magnaporthe oryzae genome encodes a large number of secreted proteins. However, the function of majority of M. oryzae secreted proteins remain to be characterized. We previously identified 851 in planta-expressed M. oryzae genes encoding putative secreted proteins, and characterized five M. oryzae cell death-inducing proteins MoCDIP1 to MoCDIP5. In the present study, we expand our work on identification of novel MoCDIP proteins. Results We performed transient expression assay of 98 more in planta-expressed M. oryzae putative secreted protein genes, and identified eight novel proteins, MoCDIP6 to MoCDIP13, that induced plant cell death. Yeast secretion assay and truncation expression analysis revealed that the signal peptides that led the secretion of proteins to the extracellular space, were required for cell death inducing activity of the novel MoCDIPs except for MoCDIP8. Exogenous treatment of rice seedlings with recombinant MoCDIP6 or MoCDIP7 resulted in enhanced resistance to blast fungus, indicating that the two MoCDIPs trigger cell death and elicit defense responses in rice. Conclusions The newly identified MoCDIP6 to MoCDIP13, together with previously identified MoCDIP1 to MoCDIP5, provide valuable targets for further dissection of the molecular mechanisms underlying the rice-blast fungus interaction.

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