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Loss of Nobox prevents ovarian differentiation from juvenile ovaries in zebrafish

文献类型： 外文期刊

作者： Qin, Mingming 1 ; Xie, Qingping 1 ; Wu, Kun 1 ; Zhou, Xianqing 5 ; Ge, Wei 1 ;

作者机构： 1.Univ Macau, Dept Biomed Sci, Taipa 999078, Macao, Peoples R China

2.Univ Macau, Fac Hlth Sci, Ctr Reprod Dev & Aging CRDA, Macau 999078, Macao, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Hydrobiol, Hangzhou 310021, Peoples R China

4.Sun Yat Sen Univ, Sch Marine Sci, State Key Lab Biocontrol, Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou 510275, Peoples R China

5.Capital Med Univ, Sch Publ Hlth, Dept Toxicol & Hyg Chem, Beijing 100069, Peoples R China

关键词： zebrafish; sex reversal; gonadal differentiation; CRISPR; Cas9; Nobox

期刊名称：BIOLOGY OF REPRODUCTION （ 影响因子：4.161； 五年影响因子：4.522 ）

ISSN： 0006-3363

年卷期： 2022 年 106 卷 6 期

页码：

收录情况： SCI

摘要： As a species without master sex-determining genes, zebrafish displays high plasticity in sex differentiation, making it an excellent model for studying the regulatory mechanisms underlying gonadal differentiation and gametogenesis. Despite being a gonochorist, zebrafish is a juvenile hermaphrodite that undergoes a special phase of juvenile ovary before further differentiation into functional testis and ovary. The mechanisms underlying juvenile ovary formation and subsequent gonadal differentiation remain largely unknown. In this study, we explored the role of Nobox/nobox (new born ovary homeobox protein), another oocyte-specific transcription factor in females, in early zebrafish gonadogenesis using CRISPR/Cas9 technology. As in mammals, nobox is specifically expressed in zebrafish gonads with a dimorphic pattern at juvenile stage. In contrast to the mutant of figla (factor in the germline alpha, another oocyte-specific transcription factor), the nobox mutants showed formation of typical perinucleolar (PN) follicles at primary growth (PG) stage in juvenile gonads, suggesting occurrence of follicle assembly from cystic oocytes (chromatin nucleolar stage, CN). These follicles, however, failed to develop further to form functional ovaries, resulting in all-male phenotype. Despite its expression in adult testis, the loss of nobox did not seem to affect testis development, spermatogenesis and male spawning. In summary, our results indicate an important role for Nobox in zebrafish ovarian differentiation and early folliculogenesis.

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