您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Using Subtracted AFLP to Efficiently Mark an Alien Chromosome Fragment in Wheat Background

文献类型： 外文期刊

作者： Chai, JF 1 ; Wu, ZM 2 ; Zhao, H 2 ; Laroche, A 2 ; Wang, HB 2 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Physiol Biochem Inst, Shijiazhuang 050051, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Physiol Biochem Inst, Shijiazhuang 050051, Peoples R China; Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada

关键词： subtracted AFLP;subtractive hybridization;AFLP;alien chromosome fragment;wheat

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Amplified fragment length polymorphism (AFLP) is a DNA fingerprinting technique (Vos et al, 1995). This technique is based on the detection of genomic restriction fragments by PCR amplification. Fingerprints are produced without prior sequence knowledge using a limited set of generic primers. By combining the reliability of the RFLP technique and the power of the PCR technique, the AFLP technique is robust and reliable, allowing the researcher to simultaneously evaluate more than 50 potential polymorphisms on a single denaturing polyacrylamide gel. This technique has been used successfully for identification of markers linked to many genes of interest (Parker et al, 1998; Bai and Kolb, 1999; Haiti et al, 1999; Huang et al, 2000). However this technique is not problem-free. When using AFLP to search for molecular markers with near-isogenic lines or bulked segregant analysis, it is easy to observe that the majority of amplified fragments is ubiquitous. In order to get a closely linked marker, usually a large number of primer pairs are needed to be screened (Huang et al, 2000). If a specific fragment is the same length as a ubiquitous fragment or just near to a strong common band, it will be more difficult for the specific band to be identified. Up to now, these problems have been paid no attention to.

  • 相关文献

[1]Identification of markers linked to major gene loci involved in determination of fruit shape index of apples (Malus domestica). Sun, Huan Huan,Wang, Yi,Zhang, Xin Zhong,Han, Zhen Hai,Zhao, Yong Bo,Li, Chun Min,Chen, Dong Mei. 2012

[2]Infraspecific Classification of Ziziphus jujuba Mill. Using AFLP Marker Technique. Bai, R. X.,Peng, J. Y.,Li, L.,Zhang, Y.,Li, X. Y.. 2009

[3]Detection of Cochliobolus heterostrophus races in South China. Wang, Meng,Wang, Shaoqing,Yu, Chuanjin,Gao, Jinxin,Chen, Jie,Wang, Meng,Wang, Shaoqing,Yu, Chuanjin,Gao, Jinxin,Chen, Jie,Ma, Jia.

[4]Inheritance and Molecular Marker of Resistance to Bot Canker in Malus domestica. Liu Hai-tao,Zhang Yu-jing,Wang Yi,Zhang Xin-zhong,Han Zhen-hai,Li Cai-li,Li Chun-min,Zhao Yong-bo,Chen Dong-mei,Li Cai-li. 2011

[5]SSR和AFLP分析玉米遗传多样性的研究. 杜金友,黎裕,王天宇,石云素,宋燕春,王海波. 2003

[6]河北省棉花枯萎菌遗传多样性及致病力分化. 高慧,王晓光,郭庆港,李社增,鹿秀云,马平. 2014

[7]棉花枯萎病菌AFLP分子标记体系的建立及初步应用. 王晓光,郭庆港,么奕清,李社增,鹿秀云,李宝庆,马平. 2011

[8]与梨黑星病抗性基因连锁的AFLP标记筛选及SCAR标记转化. 张树军,张绍铃,吴俊,王迎涛,李勇,李晓. 2010

[9]AFLP在食用豆类作物种质资源遗传多样性研究中的应用. 高倩,任钢,蔡海燕,李敏. 2008

[10]利用减法AFLP有效标记小麦中的外源染色体片段(英文). 柴建芳,吴志明,赵和,Andre LAROCHE,王海波. 2003

[11]Generation of transgenic wheat lines with altered expression levels of 1Dx5 high-molecular weight glutenin subunit by RNA interference. Yue, S. J.,Li, Y. W.,Zhu, Y. F.,Chen, Y.,Jia, X.,Li, H.,Yue, S. J.. 2008

[12]Molecular cytogenetic analysis of intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture. Li, HJ,Guo, BH,Li, YW,Du, LQ,Jia, X,Chu, CC. 2000

[13]Effects of the wheat UDP-glucosyltransferase gene TaUGT-B2 on Agrobacterium-mediated plant transformation. Zhou, Xiaohong,Wang, Ke,Du, Lipu,Lin, Zhishan,Ye, Xingguo,Liu, Yongwei.

[14]Homoeologous cloning of omega-secalin gene family in a wheat 1BL/1RS translocation. Chai, JF,Liu, X,Jia, JZ. 2005

[15]EFFECTS OF SOIL DROUGHT STRESS ON PLANT REGENERATION EFFICIENCY AND ENDOGENOUS HORMONE LEVELS OF IMMATURE EMBRYOS IN WHEAT (&ITTRITICUM AESTIVUM&IT L.). Bie, Xiaomin,Wang, Ke,Liu, Chang,Du, Lipu,Mao, Xinguo,Ye, Xingguo,Bie, Xiaomin,Liu, Yongwei. 2017

[16]Fate of labeled urea-N-15 as basal and topdressing applications in an irrigated wheat-maize rotation system in North China Plain: I winter wheat. Jia, Shulong,Wang, Xiaobin,Dai, Kuai,Zhao, Quansheng,Zhang, Xiaoming,Zhang, Dingchen,Feng, Zonghui,Wu, Xueping,Cai, Dianxiong,Jia, Shulong,Wang, Xiaobin,Dai, Kuai,Zhao, Quansheng,Zhang, Xiaoming,Zhang, Dingchen,Feng, Zonghui,Wu, Xueping,Cai, Dianxiong,Jia, Shulong,Yang, Yunma,Meng, Chunxiang,Sun, Yanming,Grant, Cynthia.

[17]Diversity of Growth Habits and Their Association with VRN Allele of 81 American Wheat Lines. Ji Gui-su,Zhang Qing-jiang,Bai Gui-hua. 2010

[18]Identification and characterization of a high kernel weight mutant induced by gamma radiation in wheat (Triticum aestivum L.). Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Cheng, Xuejiao,Chai, Lingling,Chen, Zhaoyan,Xu, Lu,Zhai, Huijie,Peng, Huiru,Yao, Yingyin,You, Mingshan,Sun, Qixin,Ni, Zhongfu,Zhao, Aiju. 2015

[19]Cloning and characterization of the Na+/H+ antiport genes from Triticum aestivum. Wang, ZN,Zhang, JS,Guo, BH,He, SJ,Tian, AG,Chen, SY. 2002

[20]Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase (BADH). Guo, BH,Zhang, YM,Li, HJ,Du, LQ,Li, YX,Zhang, JS,Chen, SY,Zhu, ZQ. 2000

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台