A novel COOH-GO-COOH-MWNT/pDA/AuNPs based electrochemical aptasensor for detection of AFB(1)
文献类型： 外文期刊
作者： Wang, Pengfei 1 ; Luo, Bin 1 ; Liu, Ke 1 ; Wang, Cheng 1 ; Dong, Hongtu 1 ; Wang, Xiaodong 1 ; Hou, Peichen 1 ; Li, Aixue 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Intelligent Equipment Res Ctr, Beijing 100097, Peoples R China
2.Jiangsu Univ, Coll Agr Engn, Zhenjiang 212000, Jiangsu, Peoples R China
期刊名称：RSC ADVANCES （ 影响因子：4.036； 五年影响因子：3.748 ）
ISSN：
年卷期： 2022 年 12 卷 43 期
页码：
收录情况： SCI
摘要： Aflatoxin B-1 (AFB(1)), one of the most common mycotoxins in food matrixes, has been identified as the most toxic contaminant with mutagenic, teratogenic, immunosuppressive, and carcinogenic effects. In this study, an electrochemical aptamer sensor was developed for the on-site detection of AFB(1). Carboxylated graphene oxide (COOH-GO) and carboxylated multi-walled carbon nanotubes (COOH-MWNT) nanocomposites, dopamine polymers (pDA) and gold nanoparticles (AuNPs) were used to enhance the electrochemical activity and the biocompatibility of the screen-printed electrodes (SPE). Once AFB(1) was captured by the aptamer immobilized on the electrode surface, the redox current of [Fe(CN)(6)](3-/4-) decreased. Therefore, the binding of aptamer (Apt) and AFB(1) can be reflected by the change of the peak current. The as-prepared sensor showed a wide detection range of 0.1 fg ml(-1)-100 pg ml(-1) and a low detection limit of 15.16 ag ml(-1). It is also simple and low-cost, which shows great potential in practical application.
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