文献类型： 外文期刊

作者： Bettoni, Jean Carlos ¹ ; Wang, Min-Rui ² ; Li, Jing-Wei ³ ; Fan, Xudong ⁴ ; Fazio, Gennaro ⁵ ; Hurtado-Gonzales, Oscar P. ⁶ ; Volk, Gayle M. ⁷ ; Wang, Qiao-Chun ⁸ ;

作者机构： 1.35 Brasil Correia St, BR-89560510 Videira, SC, Brazil

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou 571737, Peoples R China

3.Guizhou Univ, Inst Vegetable Ind Technol Res, Guiyang 550025, Peoples R China

4.Inst Pomol CAAS, Natl Ctr Eliminating Viruses Deciduous Fruit Trees, Xingcheng 125100, Peoples R China

5.USDA ARS, Plant Genet Resources Unit, Geneva, NY 14456 USA

6.USDA, APHIS Plant Germplasm Quarantine Program, BARC East, Beltsville, MD 20705 USA

7.USDA ARS, Natl Lab Genet Resources Preservat, Ft Collins, CO 80521 USA

8.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China

关键词： chemotherapy; cryotherapy; micrografting; shoot tip culture; thermotherapy; virus and viroid detection

期刊名称：PHYTOPATHOLOGY （ 影响因子：3.2； 五年影响因子：3.9 ）

ISSN： 0031-949X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Sustainable production of pome fruit crops is dependent upon having virus-free planting materials. The production and distribution of plants derived from virus- and viroid-negative sources is necessary not only to control pome fruit viral diseases but also for sustainable breeding activities, as well as the safe movement of plant materials across borders. With variable success rates, different in vitro-based techniques, including shoot tip culture, micrografting, thermotherapy, chemotherapy, and shoot tip cryotherapy, have been employed to eliminate viruses from pome fruits. Higher pathogen eradication efficiencies have been achieved by combining two or more of these techniques. An accurate diagnosis that confirms complete viral elimination is crucial for developing effective management strategies. In recent years, considerable efforts have resulted in new reliable and efficient virus detection methods. This comprehensive review documents the development and recent advances in biotechnological methods that produce healthy pome fruit plants.