您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-wide analysis of fatty acid desaturase (FAD) gene family in Camellia sinensis: Identification, expression and their biochemical functions in low temperature resistance

文献类型： 外文期刊

作者： Jin, Ya 1 ; Wang, Yingqi 1 ; Lu, Qinhua 2 ; Ren, Ning 2 ; Liu, Liping 3 ; Shen, Guoxin 2 ; Li, Da 2 ; Li, Qingsheng 2 ;

作者机构： 1.Zhejiang A&F Univ, Hangzhou 311300, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Sericulture & Tea, Hangzhou 310021, Peoples R China

3.Huzhou Acad Agr Sci, Huzhou 313000, Peoples R China

关键词： Camellia sinensis; FAD gene family; Sphingolipid Delta 8 desaturase; Low temperature

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2024 年 222 卷

页码：

收录情况： SCI

摘要： Fatty acid desaturase (FAD) is critical for unsaturated fatty acids (UFAs) biosynthesis and plays crucial roles in plants' response to low temperature. Nevertheless, a comprehensive genomic analysis of the FAD gene family in Camellia sinensis remain unreported. This study employed bioinformatics to identify 17 CsFAD genes, which are unequally distributed across 9 chromosomes. CsFAD proteins are localized to chloroplasts and/or endoplasmic reticulum (ER) by subcellular localization predictions. Phylogenetic categorization divides CsFADs into six subfamilies. The cis-elements are related to hormonal regulation and adversity response. Additionally, differential expression patterns of CsFADs across various tissues were observed. qRT-PCR analysis revealed a marked upregulation in the expression of CsSLD3 and CsSLD4 (encoding sphingolipid Delta 8 desaturase) under cold stress conditions. Moreover, subcellular localization assays in tobacco leaves confirmed the presence of CsSLD3 and CsSLD4 within ER. Yeast heterologous expression demonstrated the capacity of CsSLD3/4 to transform ceramide (t18:0) into ceramide(t18:1), and overexpression of CsSLD3/4 enhanced yeast tolerance to low temperature. Thus, this comprehensive analysis of the CsFAD gene family furnishes a robust foundation for future study into the biological functions of CsFAD genes, and offers a profound insight into their regulatory roles in stress resilience.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台