Functional analysis of AobHLH88 involved in anthocyanin synthesis using multi-omics data in Asparagus officinalis
文献类型： 外文期刊
作者： Ying, Jiali 1 ; Wen, Shuangshuang 1 ; Cai, Yunfei 1 ; Ye, Youju 1 ; Li, Lebin 2 ; Qian, Renjuan 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, 334 Xueshan Rd, Wenzhou 325005, Zhejiang, Peoples R China
2.Wenzhou Shenlu Seeds Co Ltd, Wenzhou 325005, Zhejiang, Peoples R China
关键词： Asparagus officinalis; AobHLH88; Anthocyanin synthesis
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 312 卷
页码：
收录情况： SCI
摘要： Basic helix loop helix (bHLH) proteins play critical functions in various aspects of plant growth and development, especially in anthocyanin synthesis. Although the Asparagus genome has been sequenced, there is limited information on the bHLH gene family within this species. This study identifies 96 AobHLH transcription factor genes in the Asparagus genome. Chromosomal localization indicated 91 AobHLHs across nine chromosomes. Phylogenetic analysis categorized them into nine distinct subfamilies, each with specific motif characteristics. Synteny analysis showed that both dispersed duplication and whole-genome or segmental duplications contributed to the expansion of AobHLHs, identifying 19 orthologous pairs between Asparagus and Arabidopsis. Differential expression analysis indicated a notable increase in AobHLH10, AobHLH18, and AobHLH88 gene expression during anthocyanin synthesis, as shown in Asparagus RNA-seq data. Among them, AobHLH88 displayed the higher differential expression pattern in 'Purple Passion'. Subcellular localization analysis displayed that AobHLH88 was localized in the nucleus. Furthermore, overexpression of AobHLH88 in tobacco plants highlighted its significant role in enhancing anthocyanin accumulation in Asparagus spear color. Collectively, this in-depth identification and characterization of AobHLH gene family members establish a foundational resource for future investigations on the molecular mechanism involvement of bHLH genes in Asparagus.
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