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Xyloglucan endotransglucosylase-hydrolase 1 is a negative regulator of drought tolerance in barley via modulating lignin biosynthesis and stomatal closure

文献类型： 外文期刊

作者： Fu, Man-Man 1 ; Cao, Fangbin 2 ; Qiu, Cheng-Wei 2 ; Liu, Chen 2 ; Tong, Tao 4 ; Feng, Xue 2 ; Cai, Shengguan 2 ; Chen, Zhong-Hua 5 ; Wu, Feibo 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China

2.Zhejiang Univ, Coll Agr & Biotechnol, Zijingang Campus, Hangzhou 310058, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Yangzhou 225009, Peoples R China

4.Yangtze Univ, Coll Agr, Collaborat Innovat Ctr Grain Ind, Jingzhou, Peoples R China

5.Western Sydney Univ, Sch Sci, Penrith, NSW 2751, Australia

6.Western Sydney Univ, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia

关键词： Barley transformation; Hordeum vulgare; Xyloglucan endotransglucosylase/hydrolase; Lignin; Phenylpropanoid pathway; Transcriptomics; Metabolomics

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2024 年 216 卷

页码：

收录情况： SCI

摘要： The projected increase in drought severity and duration worldwide poses a significant threat to crop growth and sustainable food production. Xyloglucan endotransglucosylase/hydrolases (XTHs) family is essential in cell wall modification through the construction and restructuring of xyloglucan cross-links, but their role in drought tolerance and stomatal regulation is still illusive. We cloned and functionally characterized HvXTH1 using genetic, physiological, biochemical, transcriptomic and metabolomic approaches in barley. Evolutionary bioinformatics showed that orthologues of XTH1 was originated from Streptophyte algae (e.g. some species in the Zygnematales) the closest clade to land plants based on OneKP database. HvXTH1 is highly expressed in leaves and HvXTH1 is localized to the plasma membrane. Under drought conditions, silencing HvXTH1 in droughttolerant Tibetan wild barley XZ5 induced a significant reduction in water loss rate and increase in biomass, however overexpressing HvXTH1 exhibited drought sensitivity with significantly less drought-responsive stomata, lower lignin content and a thicker cell wall. Transcriptome profile of the wild type Golden Promise and HvXTH1-OX demonstrated that drought-induced differentially expressed genes in leaves are related to cell wall biosynthesis, abscisic acid and stomatal signaling, and stress response. Furthermore, overexpressing HvXTH1 suppressed both genes and metabolites in the phenylpropanoid pathway for lignin biosynthesis, leading to drought sensitivity of HvXTH1-OX. We provide new insight by deciphering the function of a novel protein HvXTH1 for drought tolerance in cell wall modification, stomatal regulation, and phenylpropanoid pathway for lignin biosynthesis in barley. The function of HvXTH1 in drought response will be beneficial to develop crop varieties adapted to drought.

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