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Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains

文献类型： 外文期刊

作者： Zhong, Boxiong 1 ; Li, Jianying 2 ; Chen, Jin'e 2 ; Ye, Jian 2 ; Yu, Songdong 2 ;

作者机构： 1.Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Peoples R China; Zhejiang Acad Agr Sci, Inst Sericultural Res, Hangzhou 310021, Peoples R China

关键词： Bombyx mori;piggyBac;transposon;transgenesis;transformation efficiency

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

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收录情况： SCI

摘要： The transformation rate of three different strains of silkworm Bombyx mori was compared after the introduction of enhanced green fluorescence protein (EGFP)-encoding genes into the silkworm eggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a transposase-encoding sequence.Although there were no significant differences among the three strains in the percentages of fertile moths in microinjected eggs (P=0.1258),the percentages of G0 transformed moths in fertile moths and injected eggs were both significantly different (P=0.01368 and P=0.02398,respectively).The transformation rate of the Nistari strain (Indian strain) was significantly higher than that of the other two strains,Golden-yellow-cocoon (Vietnamese strain) and Jiaqiu (Chinese strain),which had similar rate.These results indicate that the transformation efficiency of the piggyBac-based system might vary with silkworm strains with different genetic backgrounds.The presence of endogenous piggyBac-like elements might be an important factor influencing the transformation efficiency of introduced piggyBac-derived vectors,and the diverse amount and activation in different silkworm strains might account for the significant differences.

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