A cost-effective oligo-based barcode system for chromosome identification in longan and lychee
文献类型： 外文期刊
作者： Yu, Zehuai 1 ; Qi, Yiying 2 ; Wei, Yuxuan 1 ; Zhuang, Gui 1 ; Li, Yihan 1 ; Wang, Baiyu 1 ; Akbar, Sehrish 1 ; Xu, Yi 1 ; Hua, Xiuting 1 ; Xu, Qiutao 1 ; Deng, Zuhu 1 ; Zhang, Jisen 1 ; Huang, Yongji 3 ; Yu, Fan 1 ; Zhou, Jiannan 4 ;
作者机构： 1.Guangxi Univ, Guangxi Key Lab Sugarcane Biol, State Key Lab Conservat & Utilizat Subtrop Agrobio, 100 Univ East Rd, Nanning 530004, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Agr, 15 Shangxiadian Rd, Fuzhou 350002, Peoples R China
3.Minjiang Univ, Coll Geog & Oceanog, Ministerial & Prov Joint Innovat Ctr Safety Prod C, Fuzhou 350108, Peoples R China
4.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang 524091, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）
ISSN： 2662-6810
年卷期： 2025 年 12 卷 1 期
页码：
收录情况： SCI
摘要： Oligonucleotide (Oligo)-based fluorescence in situ hybridization (FISH) represents a highly effective methodology for identifying plant chromosomes. Longan is a commercially significant fruit species, yet lacking basic chromosomal markers has hindered its cytogenetic research. In this study, we developed a cost-effective oligo-based system for distinguishing chromosomes of longan (Dimocarpus longan Lour., 2n = 2x = 30). For this system, each synthesized oligo contained two chromosome-specific sequences that spanned a distance of over 200 kb, and a PCR-based flexible amplification method coupled with nested primers was used for probe labeling. The use of these oligo-based barcodes enabled the marking of 36 chromosomal regions, which allowed for the unambiguous distinction of all 15 chromosomes in both longan and lychee (Litchi chinensis Sonn., 2n = 2x = 30) species. Based on the identification of individual chromosomes, we constructed karyotypes and detected genome assembly errors involving the 35S ribosomal RNA gene (35S rDNA) in longan and lychee. Developing oligo-based barcodes offers considerable promise for advancing cytogenetic research in longan, lychee, and their related species. Furthermore, this cost-effective synthesis system can be referred to the development of new oligo libraries among other species.
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