Molecular Variation and Phylogeny of Thymidylate Kinase Genes of Candidatus Phytoplasma ziziphi from Different Resistant and Susceptible Jujube Cultivars in China
文献类型： 外文期刊
作者： Song, Chuan-Sheng 1 ; Xu, Qi-Cong 2 ; Wan, Cui-Ping 1 ; Kong, De-Zhi 3 ; Lin, Cai-Li 3 ; Yu, Shao-Shuai 4 ;
作者机构： 1.Heze Univ, Coll Agr & Biol Engn, Heze 274015, Peoples R China
2.Int Nat Farming Res Ctr, Nagano 3901401, Japan
3.Chinese Acad Forestry, Ecol & Nat Conservat Inst, Key Lab Forest Protect Natl Forestry & Grassland A, Beijing 100091, Peoples R China
4.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China
关键词： phytoplasma; host plant resistance; thymidylate kinase gene; evolutionary pathway; potential mobile unit
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2024 年 13 卷 11 期
页码：
收录情况： SCI
摘要： The thymidylate kinase (tmk) gene is indispensable for the proliferation and survival of phytoplasma. To reveal the molecular variation and phylogeny of the tmk genes of Candidatus phytoplasma ziziphi, in this study, the tmk genes of 50 phytoplasma strains infecting different resistant and susceptible jujube cultivars from different regions in China were amplified and analyzed. Two sequence types, tmk-x and tmk-y, were identified using clone-based sequencing. The JWB phytoplasma strains were classified into three types, type-X, type-Y, and type-XY, based on the sequencing chromatograms of the tmk genes. The type-X and type-Y strains contained only tmk-x and tmk-y genes, respectively. The type-XY strain contained both tmk-x and tmk-y genes. The type-X, type-Y, and type-XY strains comprised 42%, 12%, and 46% of all the strains, respectively. The type-X and type-XY strains were identified in both susceptible and resistant jujube cultivars, while type-Y strain was only identified in susceptible cultivars. Phylogenetic analysis indicated that the tmk genes of the phytoplasmas were divided into two categories: phylo-S and phylo-M. The phylo-S tmk gene was single-copied in the genome, with an evolutionary pattern similar to the 16S rRNA gene; the phylo-M tmk gene was multi-copied, related to PMU-mediated within-genome transposition and between-genome transfer. Furthermore, the phylogenetic tree suggested that the tmk genes shuttled between the genomes of the Paulownia witches' broom phytoplasma and JWB phytoplasma. These findings provide insights into the evolutionary and adaptive mechanisms of phytoplasmas.
- 相关文献
作者其他论文 更多>>
-
Beyond Single-Pathogen Models: Understanding Mixed Infections Involving Phytoplasmas and Other Plant Pathogens
作者：Yu, Shao-Shuai;Wei, Wei
关键词：'one pathogen, one disease' concept; plant pathobiome paradigm; co-infection; unculturable plant pathogen; plant disease prevention
-
Occurrence of Praxelis clematidea Witches' Broom Disease Associated with 16SrI Group 'Candidatus Phytoplasma asteris' in Hainan Island of China
作者：Yu, Shao-Shuai;Song, Wei-Wei;Song, Chuan-Sheng
关键词：'Candidatus Phytoplasma asteris'; molecular identification; phytoplasma; Praxelis clematidea; witches' broom disease
-
Occurrence of 'Candidatus Phytoplasma asteris'-Related Strains Infecting Chinaberry (Melia azedarach) Showing Chlorotic Leaf Symptoms on Hainan Island of China
作者：Yu, Shao-Shuai;Zhou, De;Song, Wei-Wei;Zhou, De
关键词：'Candidatus Phytoplasma asteris'; chinaberry (Melia azedarach); chlorotic leaf disease; molecular identification; phytoplasma
-
Molecular Identification of 'Candidatus Phytoplasma malaysianum'-Related Strains Associated with Areca catechu Palm Yellow Leaf Disease and Phylogenetic Diversity of the Phytoplasmas Within the 16SrXXXII Group
作者：Yu, Shao-Shuai;Zhu, An-Na;Song, Wei-Wei;Zhu, An-Na;Che, Hai-Yan
关键词：16SrXXXII group; Areca catechu; classification and identification; palm yellow leaf disease; phytoplasma
-
Alocasia macrorrhiza Represents a New Host of 'Candidatus Phytoplasma asteris'-Related Strains Associated with Yellows Symptoms in China
作者：Yu, Shao-Shuai;Zhu, An-Na;Song, Wei-Wei
关键词：16SrI-B subgroup; Alocasia macrorrhiza; molecular detection; phytoplasma; yellows symptoms
-
Ipomoea obscura Represents a New Host of Phytoplasma Belonging to 16SrII Group Associated with Witches' Broom Symptoms in China
作者：Yu, Shao-Shuai;Song, Wei-Wei
关键词：16SrII group; Ipomoea obscura witches' broom; molecular identification; phytoplasma
-
Occurrence of Phytoplasmas Belonging to 16SrII Group Associated with Cyanthillium cinereum Witches' Broom Disease in China
作者：Yu, Shao-Shuai;Yang, De-Jie;Song, Wei-Wei;Song, Chuan-Sheng
关键词：16SrII group; Cyanthillium cinereum witches' broom disease; molecular detection; phytoplasma; plant host
