文献类型： 外文期刊

作者： Huang, Huiming ¹ ; Grajeda, Brian ⁴ ; Ellis, Cameron C. ⁴ ; Estevao, Igor L. ⁴ ; Lee, Wen-Yee ² ;

作者机构： 1.Fujian Acad Agr Sci, Inst Subtrop Agr, Jiulong Ave, Zhangzhou 363005, Fujian, Peoples R China

2.Univ Texas El Paso, Dept Chem, 500 West Univ Ave, El Paso, TX 79968 USA

3.Univ Texas Paso, Biochem Dept, 500 West Univ Ave, El Paso, TX 79968 USA

4.Univ Texas El Paso, Dept Biol Sci, 500 West Univ Ave, El Paso, TX 79968 USA

5.Univ Texas El Paso, Border Biomed Res Ctr BBRC, 500 West Univ Ave, El Paso, TX 79968 USA

关键词： Bisphenol A; Titanium dioxide nanoparticles; Arabidopsis thaliana; Proteomics; LC-MS/MS

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：7.129； 五年影响因子：7.284 ）

ISSN： 0147-6513

年卷期： 2022 年 241 卷

页码：

收录情况： SCI

摘要： Through the applications of recycling sewage sludge to soils as nutrients, bisphenol A (BPA) and titanium dioxide nanoparticles (TiO2-NPs) are commonly found in the agricultural environment. Previous studies have reported that BPA and nanoparticles are harmful to the environment. However, the combined toxicity of both compounds is not yet understood. This work presented an in-depth proteomic analysis of Arabidopsis thaliana exposed to BPA and TiO2-NPs concurrently at environmentally relevant levels. Seeds were simultaneously treated with varying concentrations of BPA (0, 10, 100, and 1000 mu g.kg(-1)) and TiO2-NPs (0, 1, 10 and 100 mg.kg(-1)). In treatment of 1000 mu g.kg(-1) BPA and 100 mg.kg(-1) TiO2-NPs, highest seed germination rate (87.97%, p < 0.05) was observed. Shorter primary roots but more branched roots were obtained in treatments of high BPA and NPs concentrations (100, 1000 mu g.kg(-1) BPA and 10, 100 mg.kg(-1) TiO2-NPs) while no significant effects on plant height and biomass were found. In the comparative analysis, both concentration related positive and negative effects were observed, such as regulation of cell proliferation (positive), root hair elongation (positive), cellular response to oxidative stress (negative), and cell wall organization (negative). In response to the stress caused by BPA and TiO2-NPs, some proteins related to plant root development, such as CD48E, DNAJ2 and GL24, were up-regulated explaining the shorter primary root length and more branched roots. Moreover, Arabidopsis may have stimu-lated its ability of resource transportation and energy metabolism to overcome the stress and maintain or somehow enhance their growth by up-regulating proteins like TBB6, CALM1, RAA2A, G3PP2 and KASC1. Our comparative proteomics analysis also highlighted multiple biological processes that consequently lead to the stability of plant growth and its stress adaptation. The results demonstrated that applying biosolids to soil as a fertilizer may be considered as a sustainable practice.