Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae)
文献类型： 外文期刊
作者： Liang, Xiao 1 ; Chen, Qing 1 ; Wu, Chunling 1 ; Liu, Ying 1 ; Han, Zhiling 3 ; Wu, Mufeng 3 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Sanya Res Acad, Hainan Key Lab Biosafety Monitoring & Mol Breedin, Sanya 572000, Hainan, Peoples R China
3.Hainan Univ, Coll Plant Protect, Haikou 570228, Hainan, Peoples R China
关键词： Paracoccus marginatus; fatty acyl-CoA reductase gene; reference gene; cassava cultivar; transcription analysis
期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）
ISSN：
年卷期： 2021 年 21 卷 5 期
页码：
收录情况： SCI
摘要： Paracoccus marginatus (Hemiptera: Pseudococcidae), known as the papaya mealybug, could cause considerable yield loss of several plants. To date, there is no molecular-based study of P. marginatus. Fatty acyl-CoA reductases (FARs) are key enzymes involved in wax synthesis. In the present study, we cloned and characterized coding sequences (CDS) of two FAR genes from P. marginatus. The results showed that PmFAR1 and PmFAR2 CDS were 1,590 and 1,497 bp in length, respectively, and sequence analysis indicated that these two genes both had the conservative motifs belonging to FAR_C superfamily. Furthermore, seven candidate reference genes were analyzed for their expression stability by using common algorithms including comparative Delta Cq method, geNorm, NormFinder, BestKeeper, and RefFinder. Eventually, beta-actin and GAPDHwere the best reference genes in evaluating the expression of those two FAR genes. We found that PmFAR1 and PmFAR2 showed distinct expression patterns in different life stages. Moreover, the transcription of PmFAR1 and PmFAR2 in P. marginatus fed on resistant cassava cultivars was significantly lower compared with those fed on susceptible ones, indicating the potential function of FAR genes in cassava resistance to P. marginatus. The present study might help in better understanding the molecular mechanism of cassava resistance to mealybug.
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