Transcriptomic and metabolomic analysis of quality deterioration of postharvest okra fruit at different storage temperatures
文献类型： 外文期刊
作者： Qiao, Yu 1 ; Zheng, Yanyan 1 ; Watkins, Christopher B. 6 ; Zuo, Jinhua 1 ; Liu, Huan 1 ; Wang, Yaqin 1 ; Wang, Zhengrong 4 ; Ma, Lili 1 ; He, Hongju 1 ; Hu, Liping 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr, Beijing 100097, Peoples R China
2.Beijing Key Lab Fruit & Vegetable Storage & Proc, Beijing 100097, Peoples R China
3.Minist Agr & Rural Areas, Key Lab Vegetable Postharvest Proc, Beijing 100097, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Urban Agr North, Minist Agr, Beijing 100097, Peoples R China
5.Hebei Univ Engn, Sch Life Sci & Food Engn, Handan 056107, Peoples R China
6.Cornell Univ, Coll Agr & Life Sci, Sch Integrat Plant Sci, Hort Sect, Ithaca, NY 14853 USA
关键词： Okra; Texture; ABA synthesis; Flavonoids synthesis; alpha-linolenic acid metabolic
期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.4； 五年影响因子：6.9 ）
ISSN： 0925-5214
年卷期： 2024 年 218 卷
页码：
收录情况： SCI
摘要： Okra fruit have short postharvest potential and are susceptible to chilling injury (CI). To increase our understanding of the metabolism of okra fruit we have analyzed the transcriptomic and metabolomic changes of the fruit stored at 4, 10, and 22 degrees C for six days. Compared with 0 d, storage at 22 degrees C for 6 days, senescence and lignification of fruit were associated with upregulation of genes related to abscisic acid (ABA) synthesis and signal transduction (ZEP, NCED, AAO, PYR/PYL, PP2C), lignin synthesis (HCT, 4CL, CCR, CAD, F5H, COMT), and cellulose synthesis (CesA). Chilling injury of okra at 4 degrees C may be caused by the activation of the alpha-linolenic acid metabolic pathway and flavonoid synthesis pathway, compromised membrane integrity, and higher reactive oxygen species levels. Storage at 10 degrees C resulted in the downregulation of ABA synthesis, signal transduction, and lignin synthesis genes, inhibition of alpha-linolenic acid pathway metabolites, and the expression of membrane lipid degradation genes (PLD, PLA, lipase, DGK, LOX). Conversely, there was an upregulation of antioxidant enzymes (APX, AO, POD, Grx, MDHAR) and genes involved in the flavonoid synthesis pathway (C4H, CHS, CHI, F3H, ANS), and with their corresponding metabolites (pinocembrin, butin, epiafzelechin, catechin). These changes were associated with slower fruit senescence, indicating that 10 degrees C better maintained the physiological quality of okra fruit after harvest. Based on these findings, a network model was established to control the postharvest quality of okra fruit under different temperature treatments. This study explored the regulatory mechanism of okra at different temperatures at the molecular level and laid a theoretical foundation for improving the quality of postharvest okra and extending its shelf life.
- 相关文献
作者其他论文 更多>>
-
Transcriptomic and metabolomic analysis of the delay in chilling injury in green bell peppers (Capsicum annuum L.) by sodium hexametaphosphate
作者：Wang, Xuanqi;Lv, Jieyu;Xu, Xiangbin;Wang, Xuanqi;Yuan, Shuzhi;Shi, Junyan;Lv, Jieyu;Zhao, Yaqi;Lu, Hongshan;Zuo, Jinhua;Chen, Bin;Wang, Qing;Lu, Hongshan
关键词：Green bell pepper fruit; Chilling injury; Sodium hexametaphosphate; Transcriptomic profiling; Metabolite
-
Sodium nitroprusside (SNP) generated nitric oxide delays senescence of cowpea ( Vigna unguiculata (L.) Walp )
作者：Bai, Chunmei;Zhang, Fan;Ma, Lili;Fu, Anzhen;Sang, Zhaoze;Guo, Susu;Wang, Hongwei;Wang, Qing;Zuo, Jinhua;Zheng, Yanyan;Zhang, Fan;Meng, Demei;Watkins, Christopher B.;Zuo, Jinhua;Bai, Chunmei;Zhang, Fan;Ma, Lili;Fu, Anzhen;Sang, Zhaoze;Guo, Susu;Wang, Hongwei;Wang, Qing;Zuo, Jinhua;Zheng, Yanyan;Wang, Qing;Zuo, Jinhua;Zheng, Yanyan;Wang, Qing;Zuo, Jinhua;Zheng, Yanyan
关键词：Multiomics; Lignification; Ethylene; Disease resistance; Texture
-
Chlorine dioxide affects metabolism of harvested sweet corn
作者：Liu, Shiyu;Zheng, Yanyan;Ma, Lili;Bai, Chunmei;Zhou, Xinyuan;Wang, Qing;Zuo, Jinhua;Liu, Shiyu;Wu, Cai 'e;Wang, Ronghuan;Shi, Yaxing;Cai, Wantao;Xu, Tianjun;Watkins, Christopher B.
关键词：Texture; Antioxidant capacity; Disease resistance; Transcriptome; Metabolome
-
Multiomics comprehensive analysis of pre-storage low-temperature on cowpea metabolism
作者：Han, Lichun;Wang, Qing;He, Xuelian;Wang, Hongwei;Ma, Lili;Bai, Chunmei;Zuo, Jinhua;Zheng, Yanyan;Han, Lichun;Wang, Zhengrong;He, Xuelian;Sun, Da-Wen;Watkins, Christopher B.;Zuo, Jinhua
关键词：Cowpea; Low temperature pre-storage; Metabolomics; Transcriptomics; ATAC-seq; Quality
-
Ectoine maintains the flavor and nutritional quality of broccoli during postharvest storage
作者：Yue, Xiaozhen;Tian, Tian;Zhao, Yaqi;Shi, Junyan;Ran, Jie;Yuan, Shuzhi;Xu, Xiaodi;Zuo, Jinhua;Wang, Qing;Tian, Tian;Feng, Bihong;Duan, Wenhui;Zhang, Yanfeng
关键词：Sulfur metabolism; Fatty acid biosynthesis; Amino acid biosynthesis; Volatile compounds
-
Functional Characterization of CsSWEET5a, a Cucumber Hexose Transporter That Mediates the Hexose Supply for Pollen Development and Rescues Male Fertility in Arabidopsis
作者：Hu, Liping;Song, Shuhui;Cheng, Bing;Liu, Guangmin;Liu, Huan;Zhao, Xuezhi;Wang, Yaqin;He, Hongju;Tian, Jiaxing;Zhang, Feng
关键词：cucumber; hexose transporter; plasma membrane; pollen development; SWEET
-
Transcriptomic, metabolomic, and physiological analysis of two varieties of Chinese cabbage (Brassica rapa L. ssp. pekinensis) that differ in their storabilit
作者：Zhao, Keyan;Yuan, Shuzhi;Xu, Xiangbin;Shi, Junyan;Zuo, Jinhua;Yue, Xiaozhen;Su, Tongbing;Wang, Qing;Zhao, Keyan;Zhu, Xiaoqian;Xu, Xiangbin;Zhao, Keyan;Zhu, Xiaoqian;Yuan, Shuzhi;Xu, Xiangbin;Shi, Junyan;Zuo, Jinhua;Yue, Xiaozhen;Su, Tongbing;Wang, Qing
关键词：Chinese Cabbage; Metabolomic; Transcripomic; Ethylene; Chlorophyll; Methyl jasmonate
