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Co-substrate fermentation of lignocellulose with added glucose and xylose to increase production of poly (β-l-malic acid)

文献类型： 外文期刊

作者： Zhang, Yutian 1 ; Zeng, Dongdong 2 ; Li, Jianing 1 ; Ma, Xiaojun 2 ; Li, Dongna 2 ; Li, Yachao 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, State Key Lab Breeding Base Cultivat & Physiol Tro, Minist Agr,Key Lab Biol & Genet Resource Utilizat, Haikou 571101, Peoples R China

2.Tianjin Univ Sci & Technol, Coll Light Ind Sci & Engn, Tianjin 300457, Peoples R China

关键词： Poly (beta-l-malic acid); Lignocellulosic hydrolysate; VB6; Co-fermentation; Aureobasidium pullulans

期刊名称：CELLULOSE （ 影响因子：4.8； 五年影响因子：5.6 ）

ISSN： 0969-0239

年卷期： 2025 年 32 卷 7 期

页码：

收录情况： SCI

摘要： Co-substrate fermentation has become an essential approach to achieving high-efficiency and low-cost production of poly (beta-l-malic acid) (PMA). Herein, sustainable rubberwood (Hevea brasiliensis) hydrolysate (RWH) was co-fermented by A. pullulans to synthesize PMA using glucose and xylose as co-substrate carbon sources. Moreover, vitamin B was explored as nutrient supplementation in this study. Results showed that compared with the single RWH substrate, the PMA production of co-substrate fermentation increased by 38-52%. Furthermore, the addition of VB6 promoted the utilization of substrates and shortened the fermentation cycle of PMA. During the scale-up fermentation, the PMA yield reached 0.50-0.54 g/g. Compared with traditional glucose culture medium, co-substrate fermentation improved the conversion rate of carbon sources and reduced PMA production cost by 14.9-36.6%. This work provides a new path for high-efficiency and economical industrial fermentation technology of PMA.

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