文献类型： 外文期刊
作者： Fu, Huanru 1 ; Chen, Shuyu 1 ; Zhang, Jinpeng 1 ; Su, Jinbo 1 ; Miao, Zhongwei 1 ; Huang, Yu 1 ; Wan, Chunhe 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fujian Anim Dis Control Technol Dev Ctr, Fujian Key Lab Avian Dis Control & Prevent, Fuzhou 350013, Peoples R China
2.Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China
3.Fujian Agr & Forestry Univ, Sch Future Technol, Fuzhou 350002, Peoples R China
关键词： goose megrivirus; TaqMan real-time PCR technology; epidemiological investigation; vertical; transmission
期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0032-5791
年卷期： 2024 年 103 卷 5 期
页码：
收录情况： SCI
摘要： The aim of this study was to develop an efficient and accurate platform for the detection of the newly identified goose megrivirus (GoMV). To achieve this goal, we developed a TaqMan real-time PCR technology for the rapid detection and identifica- tion of GoMV. Our data showed that the established TaqMan real-time PCR assay had high sensitivity, with the lowest detection limit of 67.3 copies/mL. No positive signal can be observed from other goose origin viruses (including AIV, GPV, GoCV, GHPyV, and GoAstV), with strong specificity. The coefficients of variation of repeated intragroup and intergroup tests were all less than 1.5%, with excellent repeatability. Clinical sample investigation data from domestic Minbei White geese firstly provided evidence that GoMV can be transmitted both horizontally and vertically. In conclusion, since the TaqMan real-time PCR method has high sensitivity, specificity, and reproducibility, it can be a useful candidate tool for GoMV epidemiological investigation
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