The diurnal emission of floral scent in Oncidium hybrid orchid is controlled by CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) through the direct regulation on terpene synthase
文献类型： 外文期刊
作者： Yeh, Chao-Wei 1 ; Zhong, Hui-Qin 2 ; Ho, Yung-Feng 1 ; Tian, Zhi-Hong 3 ; Yeh, Kai-Wun 1 ;
作者机构： 1.Natl Taiwan Univ, Coll Life Sci, Inst Plant Biol, Roosevelt Rd, Taipei 106, Taiwan
2.Fujian Acad Agr Sci, Fujian Engn Res Ctr Characterist Floriculture, Crop Res Inst, Fuzhou, Fujian, Peoples R China
3.Yangtze Univ, Coll Life Sci, Hubei Key Lab Waterlogging Disaster & Agr Use Wet, Jingzhou 434025, Hubei, Peoples R China
4.Natl Taiwan Univ, Ctr Weather Climate & Disaster Res, Taipei 106, Taiwan
关键词： Floral scent; Circadian rhythm; Oncidium Sharry Baby; CIRCADIAN CLOCK ASSOCIATED 1 (CCA1); CCA1 binding site (CBS)
期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.26； 五年影响因子：5.761 ）
ISSN： 1471-2229
年卷期： 2022 年 22 卷 1 期
页码：
收录情况： SCI
摘要： Background To adapt the periodic fluctuation of environmental factors, plants are subtle to monitor the natural variation for the growth and development. The daily activities and physiological functions in coordination with the natural variation are regulated by circadian clock genes. The circadian emission of floral scents is one of the rhythmic physiological activities controlled by circadian clock genes. Here, we study the molecular mechanism of circadian emission pattern of ocimene and linalool compounds in Oncidium Sharry Baby (Onc. SB) orchid. Results GC-Mass analysis revealed that Onc. SB periodically emitted ocimene and linalool during 6 to 14 o'clock daily. Terpene synthase, one of the key gene in the terpenoid biosynthetic pathway is expressed in coordination with scent emission. The promoter structure of terpene synthase revealed a circadian binding sequence (CBS), 5'-AGATTTTT-3' for CIRCADIAN CLOCK ASSOCIATED1 (CCA1) transcription factor. EMSA data confirms the binding affinity of CCA1. Transactivation assay further verified that TPS expression is regulated by CCA1. It suggests that the emission of floral scents is controlled by CCA1. Conclusions The work validates that the mechanism of circadian emission of floral scents in Onc. Sharry Baby is controlled by the oscillator gene, CCA1(CIRCADIAN CLOCK ASSOCIATED 1) under light condition. CCA1 transcription factor up-regulates terpene synthase (TPS) by binding on CBS motif, 5'-AGATTTTT-3' of promoter region to affect the circadian emission of floral scents in Onc. SB.
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