Cloning, Expression, and Functional Characterization of Two Highly Efficient Flavonoid-di-O-glycosyltransferases ZmUGT84A1 and ZmUGT84A2 from Maize (Zea mays L.)
文献类型： 外文期刊
作者： Sun, Xiaorong 1 ; Ke, Zhao 1 ; Zheng, Dengyu 1 ; She, Meng 1 ; Wu, Zhongyi 1 ; Li, Qing X. 4 ; Zhang, Zhongbao 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Biotechnol, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing 100097, Peoples R China
2.Beijing Univ Agr, Coll Plant Sci & Technol, Beijing 100096, Peoples R China
3.Yangtze Univ, Coll Agr, Jingzhou 434022, Hubei, Peoples R China
4.Univ Hawaii Manoa, Dept Mol Biosci & Bioengn, 1955 East West Rd, Honolulu, HI 96822 USA
关键词： flavonoid-di-O-glycosyltransferase; ZmUGT84A1; ZmUGT84A2; glycoxidation; glycoside; maize
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 13 期
页码：
收录情况： SCI
摘要： The maize (Zea mays L.) glycosyltransferase family 1 comprises many uridine diphosphate glycosyltransferase (UGT) members. However, UGT activities and biochemical functions have seldom been revealed. In this study, the genes of two flavonoid di-O-glycosyltransferases ZmUGT84A1 and ZmUGT84A2 were cloned from maize plant and expressed in Escherichia coli. Phylogenetic analysis showed that the two enzymes were homologous to AtUGT84A1 and AtUGT84A3. The two recombinant enzymes showed a high conversion rate of luteolin to its glucosides, mainly 4 ',7-di-O-glucoside and minorly 3 ',7-di-O-glucoside in two-step glycosylation reactions in vitro. Moreover, the recombinant ZmUGT84A1 and ZmUGT84A2 had a broad substrate spectrum, converting eriodictyol, naringenin, apigenin, quercetin, and kaempferol to monoglucosides and diglucosides. The highly efficient ZmUGT84A1 and ZmUGT84A2 may be used as a tool for the effective synthesis of various flavonoid O-glycosides and as markers for crop breeding to increase O-glycosyl flavonoid content in food.
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