您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Monoclonal antibody-based indirect competitive ELISA for quantitative detection of Enterobacteriaceae siderophore enterobactin

文献类型： 外文期刊

作者： Cui, Yifang 1 ; Wang, Huiwen 2 ; Guo, Fangfang 1 ; Cao, Xiaoya 1 ; Wang, Xue 1 ; Zeng, Ximin 2 ; Cui, Guolin 3 ; Lin, Jun 2 ; Xu, Fuzhou 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing 100097, Peoples R China

2.Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA

3.Hebei Univ Engn, Coll Life Sci & Food Engn, Handan 056038, Peoples R China

关键词： Enterobacteriaceae; Enterobactin; Monoclonal antibody; ic-ELISA

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 391 卷

页码：

收录情况： SCI

摘要： Enterobactin (Ent) is a promising indicator to monitor intestinal level of Enterobacteriaceae for assessment of gut inflammation. In this study, we developed a monoclonal antibody (mAb)-based ELISA for Ent quantification. We immunized mice with an Ent conjugate vaccine. An mAb named 2E4, with the highest anti-Ent antibody titer, was selected for developing indirect competitive ELISA (ic-ELISA). The purified mAb 2E4 showed high affinity (3.1 x 10(-10) M) and specificity to Ent. The limit of detection of ic-ELISA was 0.39 mu g/mL. The intra-and inter assay recovery rates of standard curve were up to 94.6% with the coefficients of variation between 4.0% and 12.3%, indicating high accuracy, repeatability, and reproducibility of the ic-ELISA. In addition, the ic-ELISA was able to quantitatively detect Ent produced in different bacterial cultures. Collectively, this study developed an icELISA with excellent performance in Ent quantification, laying a solid foundation for Ent-based diagnostics of gut health.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台