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Immune responses and protective efficacy of American eel (Anguilla rostrata) immunized with a formalin-inactivated vaccine against Anguillid herpesvirus

文献类型： 外文期刊

作者： Zhang, Li -Juan 1 ; Chen, Qiang 1 ; Yang, Jin-Xian 1 ; Ge, Jun-Qing 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Biotechnol, Wusi Rd 247, Fuzhou 350003, Peoples R China

关键词： Anguillid herpesvirus; Anguilla rostrata; Formalin-inactivated vaccine; Immune response; Protective efficacy

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 144 卷

页码：

收录情况： SCI

摘要： Anguillid herpesvirus 1 (AngHV), the causative agent of "mucus sloughing and hemorrhagic septicemia disease", causes serious infectious diseases in farmed eel. Among the effective prevention and control strategies, vaccination is one of the most effective approaches. However, no vaccine for AngHV is available. Our study developed a formalin-inactivated AngHV vaccine and evaluated its performance in American eels. Initially, AngHV-FJ, a strain of AngHV, was inactivated completely by 0.1 % formaldehyde, mixed with adjuvant Montanide ISA 763 A VG (763A). Then, vaccines containing different amount of antigen (3 x 10(6) PFU, 3 x 10(5) PFU, 3 x 10(4) PFU, 3 x 10(3) PFU) were immunized in each American eels. The results showed that the 3 x 10(5) PFU/fish was the proper dose. The inactivated AngHV vaccine was proven safe for American eels by back intramuscular injection. The results of twice immunization showed that antibody production peaked in the 8th week after the first immunization, and the antibody titer was 1:64,000. Furthermore, the immunized fishes challenged with AngHV (105 PFU/ml immersion) showed a significantly lower incidence rate (33.33 %) than the control group (95.65 %). The survival of the fish in the vaccine group (94.44 %) was significantly higher than the control group (60.87 %). The relative survival rate of the vaccinated group was 85.80 %. Also, vaccine group tissue collected at 7th d postchallenge showed reduced tissue damage and a lower virus load than the control group. The expression of cytokines of IL-1 beta, IFN-alpha, IFN-gamma, Mx1, RIG-1, and IRF-3, were significantly lower in the vaccine group than the control group at the 7th and 14th d post-challenge. Overall, the formalin-inactivated AngHV vaccine was safe and had immune protective effects against AngHV infection.

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