Role of RNA Modifications, Especially m6A, in Aflatoxin Biosynthesis of Aspergillus flavus
文献类型： 外文期刊
作者： Yang, Chi 1 ; Wu, Dandan 1 ; Lin, Hong 1 ; Ma, Dongmei 1 ; Fu, Wangzhuo 1 ; Yao, Yanfang 1 ; Pan, Xiaohua 1 ; Wang, Shihua 1 ; Zhuang, Zhenhong 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Key Lab Biopesticide & Chem Biol, Educ Minist,Prote Res Ctr, Fuzhou 350002, Peoples R China
2.Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China
3.Fujian Acad Agr Sci, Inst Edible Mushroom, Fuzhou 350012, Peoples R China
4.Fujian Agr & Forestry Univ, Coll Anim Sci, Coll Bee Sci, Fuzhou 350002, Peoples R China
关键词： Aspergillus flavus; RNAmodifications; m6A; temperature; aflatoxin; aflQ
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 72 卷 1 期
页码：
收录情况： SCI
摘要： RNA modifications play key roles in eukaryotes, but the functions in Aspergillus flavus are still unknown. Temperature has been reported previously to be a critical environmental factor that regulates the aflatoxin production of A. flavus, but much remains to be learned about the molecular networks. Here, we demonstrated that 12 kinds of RNA modifications in A. flavus were significantly changed under 29 degrees C compared to 37 degrees C incubation; among them, m6A was further verified by a colorimetric method. Then, the transcriptome-wide m6A methylome and m6A-altered genes were comprehensively illuminated through methylated RNA immunoprecipitation sequencing and RNA sequencing, from which 22 differentially methylated and expressed transcripts under 29 degrees C were screened out. It is especially notable that AFCA_009549, an aflatoxin biosynthetic pathway gene (aflQ), and the m6A methylation of its 332nd adenine in the mRNA significantly affect aflatoxin biosynthesis in A. flavus both on media and crop kernels. The content of sterigmatocystin in both Delta aflQ and aflQ(A332C) strains was significantly higher than that in the WT strain. Together, these findings reveal that RNA modifications are associated with secondary metabolite biosynthesis of A. flavus.
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