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Enhancing stimulation of cyaniding, GhLDOX3 activates reactive oxygen species to regulate tolerance of alkalinity negatively in cotton

文献类型： 外文期刊

作者： Jiang, Tiantian 1 ; He, Yunxin 4 ; Wu, Zhe 3 ; Cui, Yupeng 1 ; Wang, Xiuping 3 ; Huang, Hui 1 ; Fan, Yapeng 1 ; Han, Mingge 1 ; Wang, Junjuan 1 ; Wang, Shuai 1 ; Chen, Xiugui 1 ; Lu, Xuke 1 ; Wang, Delong 1 ; Guo, Lixue 1 ; Zhao, Lanjie 1 ; Hao, Fushun 2 ; Ye, Wuwei 1 ;

作者机构： 1.Chinese Acad Agr Sci, Anyang Inst Technol, Natl Key Lab Cotton Biobreeding & Integrated Utili, Res Base,Inst Cotton Res, Anyang 455000, Henan, Peoples R China

2.Henan Univ, Sch Life Sci, Natl Key Lab Cotton Biobreeding & Integrated Utili, Kaifeng 475004, Henan, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Inst Coastal Agr, Tangshan 063299, Hebei, Peoples R China

4.Hunan Inst Cotton Sci, Changde 415101, Hunan, Peoples R China

关键词： Cotton; Anthocyanins; Cyanidin; Alkali stress; GhLDOX3

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 267 卷

页码：

收录情况： SCI

摘要： Anthocyanins belong to flavonoid secondary metabolites that act as plant pigments to give flowers and fruits different colors and as "scavengers" of reactive oxygen species (ROS) to protect plants from abiotic and biotic stresses. Few studies linked anthocyanins to alkaline resistance so far. In this study, anthocyanin synthesis related gene leucoanthocyanidin dioxygenase (LDOX) was screened as a candidate gene to explore its relationship with alkali stress. The results found that pYL156: GhLDOX3 lines treated with 50 mM Na2CO3 (pH 11.11) for 24 h showed a significant increase in peroxidase (POD) activity, a decrease in total anthocyanin content and an increase in cyanidin content and a decrease in ROS accumulation compared to pYL156. The overexpressed (OE) lines, ldox mutant and wild-type (WT) lines in Arabidopsis were treated with 50 mM Na2CO3, 100 mM Na2CO3 and 150 mM Na2CO3 for 8 d, respectively. The wilted degree of the OE lines was more severe than WT lines, and less severe in the mutant lines in the 150 mM Na2CO3 treatment. After treatment, the expression levels of AtCAT and AtGSH genes related to antioxidant system in OE lines were significantly lower than in WT, and the expression levels of AtCAT and AtGSH in mutant lines were significantly higher than in WT. In conclusion, the above results suggest GhLDOX3 played a negative regulatory role in the mechanism of resisting Na2CO3 stress. Therefore, it can be considered in cotton breeding to improve the alkali tolerance of cotton by regulating the expression of related genes.

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