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Investigation of grapevine circular RNA Vv-circPAS1 revealed the function on root development and salt stress resistance

文献类型： 外文期刊

作者： Liu, Jingjing 1 ; Ren, Yi 3 ; Xu, Yuanyuan 1 ; Yin, Yonggang 4 ; Han, Bin 4 ; Zhang, Zhen 1 ; Li, Junpeng 1 ; Song, Yue 1 ; Zhang, Lipeng 2 ; Fan, Dongying 1 ; Liu, Mingying 1 ; Wang, Lujia 1 ; Teng, Yuanxu 2 ; Liu, Huaifeng 2 ; Ma, Chao 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Shanghai Collaborat Innovat Ctr Agriseeds, Sch Agr & Biol, Shanghai 200240, Peoples R China

2.Agr Coll Shihezi Univ, Dept Hort, Key Lab Special Fruits & Vegetables Cultivat Physi, Shihezi 832003, Peoples R China

3.Yunnan Agr Univ, Coll Landscape & Hort, Kunming 650201, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Changli Res Inst Fruit Trees, Changli 066600, Hebei, Peoples R China

关键词： circRNA; Grapevine; Root development; Salt tolerance

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 0304-4238

年卷期： 2024 年 337 卷

页码：

收录情况： SCI

摘要： Circular RNAs (circRNAs) exist widely in animals and plants. Most researches focused on the identification and annotation of circRNA in plants, while the biological functions of circRNA are still limited. Based on our previous whole transcriptomic analysis of grapevines, in this study, 15 circRNAs were further selected to validate in grapevine through investigating their origin and back-splicing sites. The results showed that only four circRNAs ( Vv-circRNA_0490, Vv-circRNA_1292, Vv-circRNA_2094 and Vv-circRNA_2451) ) followed the AG/GT canonical splicing signal. Moreover, we found that most of circRNAs were specifically expressed in different tissues and were preferential accumulation in cytoplasm and nucleus by nuclear-cytoplasmic fractionation assay. Furthermore, the expression cassettes of circRNAs were constructed and found that all circRNAs were precisely back- spliced in Nicotiana benthamiana, , indicating that the conserved splicing mechanism of circRNA. In addition, Vv-circPAS1 a circRNA processed from host gene VvPAS1 which is a member of the FK506-binding protein (FKBP) gene family, were focused because of association with root development. The promoter analysis of VvcircPAS1 showed that multiple cis-acting elements related to defense, light, phytohormone and plant development were found. Finally, Vv-circPAS1 was introduced into Arabidopsis and found that Vv-circPAS1 significantly inhibited the elongation of primary roots and increased the number and length of roots hair. Meanwhile, overexpression of Vv-circPAS1 improved salt tolerance in grapevine callus, while the linear fragments didn't. These results broaden our understanding of circRNAs in plants, and provide new insight into plant circRNAs.

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