LlbHLH87 interacts with LlSPT to modulate thermotolerance via activation of LlHSFA2 and LlEIN3 in lily
文献类型： 外文期刊
作者： Wu, Ze 1 ; Gong, Xue 1 ; Zhang, Yinyi 1 ; Li, Ting 1 ; Xiang, Jun 1 ; Fang, Qianqian 1 ; Yu, Junpeng 1 ; Ding, Liping 1 ; Liang, Jiahui 4 ; Teng, Nianjun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, Natl Forestry & Grassland Adm, Key Lab Landscaping,Minist Agr & Rural Affairs,Key, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Nanjing Oriole Isl Modern Agr Dev Co Ltd, Lily Sci & Technol Backyard Qixia Jiangsu, Jiangsu Grad Workstat, Nanjing 210043, Peoples R China
3.Nanjing Agr Univ, Coll Agr, Nanjing 210095, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Inst Grassland Flowers & Ecol, Beijing 100097, Peoples R China
关键词： bHLH transcription factor; heat stress; Lilium longiflorum; LlbHLH87; LlEIN3; LlHSFA2; LlSPT
期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）
ISSN： 0960-7412
年卷期： 2024 年 120 卷 4 期
页码：
收录情况： SCI
摘要： Basic helix-loop-helix (bHLH) proteins comprise one of the largest families of transcription factors in plants, which play roles in plant development, secondary metabolism, and the response to biotic/abiotic stresses. However, the roles of bHLH proteins in thermotolerance are largely unknown. Herein, we identified a heat-inducible member of the bHLH family in lily (Lilium longiflorum), named LlbHLH87, which plays a role in thermotolerance. LlbHLH87 was rapidly induced by transient heat stress, and its encoded protein was localized to the nucleus, exhibiting transactivation activity in both yeast and plant cells. Overexpression of LlbHLH87 in Arabidopsis enhanced basal thermotolerance, while silencing of LlbHLH87 in lily reduced basal thermotolerance. Further analysis showed that LlbHLH87 bound to the promoters of HEAT STRESS TRANSCRIPTION FACTOR A2 (LlHSFA2) and ETHYLENE-INSENSITIVE 3 (LlEIN3) to directly activate their expression. In addition, LlbHLH87 interacted with itself and with SPATULA (LlSPT) protein. LlSPT was activated by extended heat stress and its protein competed for the homologous interaction of LlbHLH87, which reduced the transactivation ability of LlbHLH87 for target genes. Compared with that observed under LlbHLH87 overexpression alone, co-overexpression of LlbHLH87 and LlSPT reduced the basal thermotolerance of lily to sudden heat shock, but improved its thermosensitivity to prolonged heat stress treatment. Overall, our data demonstrated that LlbHLH87 regulates thermotolerance via activation of LlEIN3 and LlHSFA2, along with an antagonistic interaction with LlSPT.
- 相关文献
作者其他论文 更多>>
-
Auxin regulates bulbil development by affecting carbohydrate metabolism in Lilium lancifolium
作者：Wang, Mengdi;Li, Jiamin;Zhang, Xuemin;Kang, Shengnan;Yu, Xiaonan;Wang, Mengdi;Li, Jiamin;Pan, Wenqiang;Sang, Qianzi;Liang, Jiahui;Zhang, Xuemin;Kang, Shengnan;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Wang, Mengdi;Li, Jiamin;Pan, Wenqiang;Sang, Qianzi;Liang, Jiahui;Zhang, Xuemin;Kang, Shengnan;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Sang, Qianzi
关键词：Lilium lancifolium; Bulbil development; Transcriptome; Auxin; Carbohydrate metabolism
-
Simultaneously improve autotrophic denitrification and membrane fouling mitigation under low sludge concentration in sulfur-fed membrane bioreactor
作者：Gao, Shanshan;Li, Ting;Geng, Mingyue;Xie, Binghan;Dong, Junliang;Tian, Jiayu;Gao, Min
关键词：Membrane bioreactor; Sulfur-driven autotrophic denitrification; Membrane fouling; Microbial assembly; Sludge concentration
-
Winter wheat harvest detection via Sentinel-2 MSI images
作者：Yue, Jibo;Yao, Yihan;Shen, Jianing;Li, Ting;Wei, Yihao;Xu, Xin;Guo, Wei;Fu, Yuanyuan;Qiao, Hongbo;Ma, Xinming;Wang, Jian;Xu, Nianxu;Feng, Haikuan;Feng, Haikuan;Lin, Yinghao;Lin, Yinghao
关键词：Wheat; maturity; harvest; monitoring; vegetation index
-
Exploring Transcriptional Regulatory Network During Direct De Novo Shoot Regeneration in Poplar
作者：Ding, Liping;Yang, Zitong;Zheng, Lin;Chen, Yajuan;Wei, Jianhua;Wang, Hongzhi
关键词：regeneration; hormone;
populus ; transcriptome -
Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium
作者：Xin, Yin;Chen, Xi;Liang, Jiahui;Wu, Jingxiang;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Xin, Yin;Wang, Shaokun;Pan, Wenqiang;Wu, Jingxiang;Wu, Jian;Chen, Xi;Yu, Xiaonan;Zaccai, Michele
关键词：
-
A novel vegetation-water resistant soil moisture index for remotely assessing soil surface moisture content under the low-moderate wheat cover
作者：Yue, Jibo;Li, Ting;Guo, Wei;Qiao, Hongbo;Li, Suju;Liu, Yang;Liu, Yang;Feng, Haikuan;Yang, Hao;Yang, Guijun;Tian, Jia;Tian, Qingjiu
关键词：SMC; Radiative transfer model; VWC; VRSMI; NSDSI
-
Winter Wheat Maturity Prediction via Sentinel-2 MSI Images
作者：Yue, Jibo;Li, Ting;Shen, Jianing;Wei, Yihao;Xu, Xin;Ma, Xinming;Qiao, Hongbo;Liu, Qian;Liu, Yang;Feng, Haikuan;Feng, Haikuan;Yang, Guijun;Yang, Hao;Feng, Haikuan;Li, Changchun;Yang, Guijun
关键词：wheat; maturity; remote sensing; crop growth stage
