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Improvement of silage characteristics of Lactobacillus salivarius HMC4 and improvement of silage quality of king grass

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文献类型：外文期刊

作者： Yang, Jinsong ¹ ; Zhao, Songsong ¹ ; Zhi, Wenbo ¹ ; Lu, Tianyu ¹ ; Qiao, Huahua ¹ ; Liu, Wei ¹ ; Dou, Ying ¹ ; Tan, Haisheng ² ; Zhou, Hanlin ³ ;

作者机构： 1.Hainan Univ, Coll Food Sci & Engn, Haikou, Hainan, Peoples R China

2.Hainan Univ, Coll Mat Sci & Engn, Haikou, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Zhanjiang Expt Stn, Zhanjiang, Peoples R China

4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Minist Agr & Rural Affairs Germplasm Resources Con, Key Lab,Minist Agr & Rural Affairs Crop Gene Resou, Danzhou, Peoples R China

关键词： protoplast fusion; silage; Lactobacillus; high-throughput sequencing; microbial communities

期刊名称：FRONTIERS IN MICROBIOLOGY （影响因子：4.5；五年影响因子：5.2 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated. The results showed that the propionic acid concentration in group C was the lowest, but the organic acid content in feed was significantly increased. Groups B and C had slightly lower crude fiber levels than group A, while groups A and C had higher levels of soluble sugars. The dynamic observation of C group showed that the nutrient composition of feed changed gradually with the extension of silage period. High-throughput sequencing revealed fluctuations in microbial composition before and after silage. Over time, Lactobacillus became the dominant strain and its numbers steadily increased.

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