您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis

文献类型： 外文期刊

作者： Xin, Shichao 1 ; Udayabhanu, Jinu 1 ; Dai, Xuemei 1 ; Hua, Yuwei 1 ; Fan, Yueting 1 ; Huang, Huasun 1 ; Huang, Tiandai 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Minist Agr & Rural Affairs, Key Lab Biol & Genet Resources Rubber Tree, Rubber Res Inst,State Key Lab Incubat Base Cultiv, Haikou 571101, Hainan, Peoples R China

2.Haikou Key Lab Innovat Seedlings Trop Plants, Haikou 571101, Hainan, Peoples R China

关键词： rubber tree; constitutive promoter; cis-acting elements; Agrobacterium-mediated transformation; protoplast transformation; transgene expression

期刊名称：FORESTS （ 影响因子：3.282； 五年影响因子：3.292 ）

ISSN：

年卷期： 2022 年 13 卷 6 期

页码：

收录情况： SCI

摘要： Hevea brasiliensis is an economically important tree species that provides the only commercial source of natural rubber. The replacement of the CaMV35S promoter by endogenous polyubiquitin promoters may be a viable way to improve the genetic transformation of this species. However, no endogenous polyubiquitin promoters in Hevea have been reported yet. Here, we identified three Hevea polyubiquitin genes HbUBI10.1, HbUBI10.2 and HbUBI10.3, which encode ubiquitin monomers having nearly identical amino acid sequences to that of AtUBQ10. The genomic fragments upstream of these HbUBI genes, including the signature leading introns, were amplified as putative HbUBI promoters. In silico analysis showed that a number of cis-acting elements which are conserved within strong constitutive polyubiquitin promoters were presented in these HbUBI promoters. Transcriptomic data revealed that HbUBI10.1 and HbUBI10.2 had a constitutive expression in Hevea plants. Semi-quantitative RT-PCR showed that these three HbUBI genes were expressed higher than the GUS gene driven by CaMV35S in transgenic Hevea leaves. All three HbUBI promoters exhibited the capability to direct GFP expression in both transient and stable transformation assays, although they produced lower protoplast transformation efficiencies than the CaMV35S promoter. These HbUBI promoters will expand the availability of promoters for driving the transgene expression in Hevea genetic engineering.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台