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RNAi-Mediated Suppression of OsBBTI5 Promotes Salt Stress Tolerance in Rice

文献类型： 外文期刊

作者： Lin, Zhimin 1 ; Yi, Xiaoyan 2 ; Ali, Muhammad Moaaz 2 ; Zhang, Lijuan 2 ; Wang, Shaojuan 2 ; Tian, Shengnan 2 ; Chen, Faxing 2 ;

作者机构： 1.Fujian Acad Agr Sci, Biotechnol Inst, Fuzhou 350003, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Peoples R China

关键词： OsBBTI5; rice; 24-epibrassinolide; salt tolerance; RNAi; transcriptome

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 2 期

页码：

收录情况： SCI

摘要： This study explores the impact of RNAi in terms of selectively inhibiting the expression of the OsBBTI5 gene, with the primary objective of uncovering its involvement in the molecular mechanisms associated with salt tolerance in rice. OsBBTI5, belonging to the Bowman-Birk inhibitor (BBI) family gene, is known for its involvement in plant stress responses. The gene was successfully cloned from rice, exhibiting transcriptional self-activation in yeast. A yeast two-hybrid assay confirmed its specific binding to OsAPX2 (an ascorbate peroxidase gene). Transgenic OsBBTI5-RNAi plants displayed insensitivity to varying concentrations of 24-epibrassinolide in the brassinosteroid sensitivity assay. However, they showed reduced root and plant height at high concentrations (10 and 100 mu M) of GA(3) immersion. Enzyme activity assays revealed increased peroxidase (POD) and superoxide dismutase (SOD) activities and decreased malondialdehyde (MDA) content under 40-60 mM NaCl. Transcriptomic analysis indicated a significant upregulation of photosynthesis-related genes in transgenic plants under salt stress compared to the wild type. Notably, this study provides novel insights, suggesting that the BBI gene is part of the BR signaling pathway, and that OsBBTI5 potentially enhances stress tolerance in transgenic plants through interaction with the salt stress-related gene OsAPX2.

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