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Surfactin and fengycin contribute differentially to the biological activity of Bacillus subtilis NCD-2 against cotton verticillium wilt

文献类型： 外文期刊

作者： Dong, Lihong 1 ; Wang, Peipei 2 ; Zhao, Weisong 2 ; Su, Zhenhe 2 ; Zhang, Xiaoyun 2 ; Lu, Xiuyun 2 ; Li, Shezeng 2 ; Ma, Ping 2 ; Guo, Qinggang 2 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Baoding 071000, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Baoding 071000, Peoples R China; Minist Agr & Rural Affairs, Key Lab IPM Crops Northern Reg North China, Baoding 071000, Peoples R China; Integrated Pest Management Ctr Hebei Prov, Baoding 071000, Peoples R China

关键词： Bacillus subtilis; Surfactin; Fengycin; Biological activity; Cotton verticillium wilt

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.857； 五年影响因子：4.141 ）

ISSN： 1049-9644

年卷期： 2022 年 174 卷

页码：

收录情况： SCI

摘要： Bacillus subtilis strain NCD-2 is a promising biocontrol agent for soil borne plant diseases. A previous study indicated that strain NCD-2 could produce surfactin and fengycin simultaneously. In this study, the exact roles of surfactin and fengycin in strain NCD-2 against cotton verticillium wilt were determined by knockout of the surfactin synthetase gene srfAA and the fengycin synthetase gene fenC. Compared to the wild-type strain NCD-2, the fengycin-deficient mutant AFenC decreased the antifungal ability toward Verticillium dahliae, but the surfactin-deficient mutant ASrfAA mainly decreased the swarming and biofilm formation abilities of strain NCD-2. Comparatively, the fengycin and surfactin double mutant AFenC/SrfAA decreased the antifungal activity and swarming and biofilm formation abilities to more significant levels. The colonization abilities of the wild type strain NCD-2 and its derivative mutants were compared in the cotton rhizosphere. Compared to the wild-type strain NCD-2, ASrfAA and AFenC/SrfAA exhibited the poorest colonization ability. RT-qPCR results confirmed that knockout of the srfAA and fenC genes decreased the expression of biofilm formation-related genes. The biocontrol abilities of the strains against cotton verticillium wilt were evaluated in a greenhouse. All mutants decreased the biocontrol abilities against cotton verticillium wilt, while AFenC/SrfAA showed the poorest biocontrol ability, followed by AFenC. Taken together, fengycin was the main antifungal active compound, and surfactin affected root colonization. Both the antifungal ability and root colonization of strain NCD-2 played important roles in suppressing cotton verticillium wilt.

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