文献类型： 外文期刊

作者： Hu, S. -C. ¹ ; Hong, K. ² ; Song, Y. -C. ¹ ; Liu, J. -Y. ¹ ; Tan, R. -X. ¹ ;

作者机构： 1.Nanjing Univ, Inst Funct Biomol, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

2.Chinese Acad Trop Agr Sci, State Key Lab Trop Crop Biotechnol, Haikou 571101, Peoples R China

3.Wuhan Polytech Univ, Dept Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China

关键词： biotransformation

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

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收录情况： SCI

摘要： A marine Streptomyces sp. 060524 capable of hydrolyzing the glycosidic bond of isoflavone glycosides, was isolated by detecting its beta-glucosidase activity. 5 isoflavone aglycones were isolated from culture filtrates in soybean meal glucose medium. They were identified as genistein (1), glycitein (2), daidzein (3), 3',4',5,7-tetrahydroxyisoflavone (4), and 3',4',7-trihydroxyisoflavone (5), based on UV, NMR and mass spectral analysis. The Streptomyces can selectively hydroxylate at the 3'-position in the daidzein and genistein to generate 3'-hydroxydaidzein and 3'-hydroxygenistein, respectively. The Strain biotransformed more than 90% of soybean isoflavone glycosides into their aglycones within 108 h. 3'-hydroxydaidzein and 3'-hydroxygenistein exhibited stronger cytotoxicity against K562 human chronic leukemia than daidzein and genistein.