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Time-Series Metabolome and Transcriptome Analyses Reveal the Genetic Basis of Vanillin Biosynthesis in Vanilla

文献类型： 外文期刊

作者： Dong, Zeyu 1 ; Zhao, Shaoguan 1 ; Xing, Yizhang 1 ; Su, Fan 1 ; Xu, Fei 1 ; Fang, Lei 2 ; Zhang, Zhiyuan 2 ; Zhao, Qingyun 1 ; Gu, Fenglin 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Natl Ctr Important Trop Crops Engn & Technol Res, Key Lab Proc Suitabil & Qual Control Special Trop, Spice & Beverage Res Inst,Hainan Key Lab Genet Imp, Hainan 571533, Peoples R China

2.Zhejiang Univ, Hainan Inst, Sanya 572025, Peoples R China

3.Zhejiang Univ, Adv Seed Inst, Zhejiang Prov Key Lab Crop Genet Resources, Key Lab Plant Factory Plant Factory Generat Adding, Hangzhou 310058, Peoples R China

4.Chinese Acad Trop Agr Sci, Sanya Res Inst, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya 572025, Peoples R China

关键词： Vanilla planifolia Andrews; vanillin; combined analysis; transcriptome and metabolome

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 13 期

页码：

收录情况： SCI

摘要： Vanillin, the principal aromatic compound in vanilla, is primarily derived from mature pods of vanilla (Vanilla planifolia Andrews). Although the biosynthetic pathway of vanillin has been progressively elucidated, the specific key enzymes and transcription factors (TFs) governing vanillin biosynthesis require further comprehensive investigation via combining transcriptomic and metabolomic analysis. For this study, V. planifolia (higher vanillin producer) and V. imperialis (lower vanillin producer) were selected. Time-series metabolomics analysis revealed 160-220 days after pollination (DAPs) as the critical phase for vanillin biosynthesis. Combined time-series transcriptome analysis revealed 984 upregulated differentially expressed genes (DEGs) in key periods, 2058 genes with temporal expression, and 4326 module genes through weighted gene co-expression network analysis (WGCNA), revealing six major classes of TFs: No Apical Meristem (NAC), Myb, WRKY, FLOWERING PROMOTING FACTOR 1-like (FPFL), DOF, and PLATZ. These TFs display strong regulatory relationships with the expression of key enzymatic genes, including P450s, COMT, and 4CL. The NAC TF family emerged as central regulators in this network, with NAC-2 (HPP92_014056) and NAC-3 (HPP92_012558) identified as key hub genes within the vanillin biosynthetic gene co-expression network. The findings of this study provide a theoretical foundation and potential target genes for enhancing vanillin production through genetic and metabolic engineering approaches, offering new opportunities for sustainable development in the vanilla industry and related applications.

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