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A particle counting immunosensor for the sensitive detection of ochratoxin A via click chemistry-mediated signal amplification

文献类型： 外文期刊

作者： Chen, Rui 1 ; Dong, Yongzhen 2 ; Li, Shuhuai 1 ; Ma, Xionghui 1 ; Pang, Chaohai 1 ; Wang, Shu 3 ; Zhang, Jiangjiang 4 ; Chen, Yiping 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr,Minist Agr & Rural Affairs, Hainan Prov Key Lab Qual & Safety Trop Fruits & Ve, Key Lab Qual & Safety Control Subtrop Fruits & Veg, Haikou 571101, Hainan, Peoples R China

2.Dalian Polytech Univ, State Key Lab Marine Food Proc & Safety Control, Dalian 116034, Liaoning, Peoples R China

3.Chinese Acad Sci, Beijing Inst Nanoenergy & Nanosyst, Beijing 100140, Peoples R China

4.Beijing Inst Technol, Minist Ind & Informat Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, Beijing 100081, Peoples R China

关键词： SiO 2 @CuO 2 nanoparticles; Particle counter; Polystyrene microspheres; Click chemistry; Ochratoxin A

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2025 年 488 卷

页码：

收录情况： SCI

摘要： The existence of ochratoxin A (OTA) in agricultural products poses significant threats to human health and environment, underscoring the critical need for its prompt and precise quantification. A particle counting immunosensor for the highly sensitive detection of OTA was presented, employing SiO2@CuO2 nanoparticles to facilitate click chemistry. The quantity of SiO2@CuO2 nanoparticles, and consequently the Cu2* concentration, can be directly altered through the immune response involving OTA. All Cu2* underwent reduction to Cu*, resulting in numerous alkyne-functionalized 4 mu m polystyrene microspheres (alkyne-PS4 mu m) attaching to the surface of individual azide-modified 1 mm polystyrene microspheres (azide-PS1mm). This attachment occurred via a Cu*-mediated click chemistry reaction between the azide and alkyne functionalities. Finally, a handheld cell counter was employed to measure the number of remaining alkyne-PS4 mu m, allowing for the calculation of OTA concentration. It showed a wide linear detection range of 50 pg/mL to 500 ng/mL, with the detection limit of 34 pg/mL and recoveries ranging from 82.60 % to 113.00 %. This assay combined the nimbleness of PS1mmwith the precise reading capacity of a handheld cell counter to discern variations in the number of petite micro- spheres, offering a promising, expedient analytical solution for the portable detection of minute contaminants in agricultural products.

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