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Genome Analysis of Multiple Polysaccharide-Degrading Bacterium Microbulbifer thermotolerans HB226069: Determination of Alginate Lyase Activity

文献类型： 外文期刊

作者： Li, Xue 1 ; Yang, Miao 2 ; Mo, Kunlian 2 ; Hu, Yonghua 2 ; Gu, Hanjie 2 ; Sun, Dongmei 1 ; Bao, Shixiang 2 ; Huang, Huiqin 2 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Agr, Daqing 163000, Heilongjiang, Peoples R China

2.Inst Trop Biosci & Biotechnol, Hainan Inst Trop Agr Resources, CATAS, Haikou 571101, Hainan, Peoples R China

3.CATAS, Zhanjiang Expt Stn, Zhanjiang 524013, Peoples R China

4.Hainan Prov Key Lab Funct Components Res & Utiliza, Haikou 571101, Hainan, Peoples R China

5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China

6.Huazhong Agr Univ, Coll Life Sci & Technol, CATAS, Wuhan 430070, Hubei, Peoples R China

关键词： Microbulbifer thermotolerans; Polysaccharide-degrading bacteria; Alginate lyase; Genome; Oligosaccharide

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.0； 五年影响因子：3.4 ）

ISSN： 1436-2228

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Polysaccharide-degrading bacteria are key participants in the global carbon cycle and algal biomass recycling. Herein, a polysaccharide lyase-producing strain HB226069 was isolated from Sargassum sp. from Qingge Port, Hainan, China. Results of the phylogenetic of the 16S rRNA gene and genotypic analysis indicated that the isolate should be classified as Microbulbifer thermotolerans. The whole genome is a 4,021,337 bp circular chromosome with a G+C content of 56.5%. Analysis of the predicted genes indicated that strain HB226069 encoded 161 carbohydrate-active enzymes (CAZymes), and abundant putative enzymes involved in polysaccharide degradation were predicted, including alginate lyase, fucosidase, agarase, xylanase, cellulase, pectate lyase, amylase, and chitinase. Three of the putative polysaccharide lyases from PL7 and PL17 families were involved in alginate degradation. The alginate lyases of strain HB226069 showed the maximum activity of 117.4 U/mL at 50 degrees C, pH 7.0, and 0.05 M FeCl3, while exhibiting the best stability at 30 degrees C and pH 7.0. The Thin Layer Chromatography (TLC) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses indicated that the alginate oligosaccharides (AOSs) degraded by the partially purified alginate lyases contained oligosaccharides of DP2-DP5 and monosaccharide while reacting for 36 h. The complete genome of M. thermotolerans HB226069 enriches our understanding of the mechanism of polysaccharide lyase production and supports its potential application in polysaccharide degradation.

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