Bulked segregant RNA-seq reveals complex resistance expression profile to powdery mildew in wild emmer wheat W762
文献类型： 外文期刊
作者： Qian, Zejun 1 ; Liu, Ruishan 1 ; Liu, Xueqing 2 ; Qie, Yanmin 3 ; Wang, Jiangchun 2 ; Yin, Yan 2 ; Xin, Qingguo 2 ; Yu, Ningning 1 ; Zhang, Jiadong 1 ; Li, Yaoxue 1 ; Li, Jiatong 1 ; Dai, Yintao 1 ; Liu, Cheng 4 ; Jin, Yuli 1 ; Ma, Pengtao 1 ;
作者机构： 1.Yantai Univ, Coll Life Sci, Yantai Key Lab Characterist Agr Bioresource Conser, Yantai, Peoples R China
2.Inst Grain & Oil Crops, Yantai Acad Agr Sci, Yantai, Peoples R China
3.Inst Cereal & Oil Crops, Hebei Acad Agr & Forestry Sci, Hebei Lab Crop Genet & Breeding, Shijiazhuang, Peoples R China
4.Crop Res Inst, Shandong Acad Agr Sci, Jinan, Peoples R China
关键词： durum wheat; powdery mildew; BSR-seq; expression profiling; DEG
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive fungal diseases threatening global wheat production. Exploring powdery mildew resistance (Pm) gene(s) and dissecting the molecular mechanism of the host resistance are critical to effectively and reasonably control this disease. Durum wheat (Triticum turgidum L. var. durumDesf.) is an important gene donor for wheat improvement against powdery mildew. In this study, a resistant durum wheat accession W762 was used to investigate its potential resistance component(s) and profile its expression pattern in responding to Bgt invasion using bulked segregant RNA-Seq (BSR-Seq) and further qRT-PCR verification. Genetic analysis showed that the powdery mildew resistance in W762 did not meet monogenic inheritance and complex genetic model might exist within the population of W762 x Langdon (susceptible durum wheat). After BSR-Seq, 6,196 consistently different single nucleotide polymorphisms (SNPs) were called between resistant and susceptible parents and bulks, and among them, 763 SNPs were assigned to the chromosome arm 7B. Subsequently, 3,653 differentially expressed genes (DEGs) between resistant and susceptible parents and bulks were annotated and analyzed by Gene Ontology (GO), Cluster of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. The potential regulated genes were selected and analyzed their temporal expression patterns following Bgt inoculation. As a result, nine disease-related genes showed distinctive expression profile after Bgt invasion and might serve as potential targets to regulate the resistance against powdery mildew in W762. Our study could lay a foundation for analysis of the molecular mechanism and also provide potential targets for the improvement of durable resistance against powdery mildew.
- 相关文献
作者其他论文 更多>>
-
Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives
作者：Wang, Jiaojiao;Zhao, Ya;Xiao, Bei;Qian, Zejun;Huang, Xiaomei;Liu, Ruishan;Zhang, Jiadong;Jin, Yuli;Ma, Pengtao;Xu, Hongxing;Qie, Yanmin;Han, Ran;Liu, Cheng;Sun, Xiaohui
关键词：powdery mildew; markers; resistance identification; haplotype analysis; wheat relatives
-
Characterization of the powdery mildew resistance locus in wheat breeding line Jimai 809 and its breeding application
作者：Zhao, Ya;Xiao, Bei;Wang, Jiaojiao;Qian, Zejun;Huang, Xiaomei;Liu, Ruishan;Zhang, Jiadong;Jin, Yuli;Ma, Pengtao;Han, Guohao;Qie, Yanmin;Song, Jianmin;Zi, Yan;Song, Lihong
关键词：Wheat powdery mildew; PmJM809; Molecular mapping; Marker-assisted selection
-
Genetic basis of an elite wheat cultivar Guinong 29 with harmonious improvement between multiple diseases resistance and other comprehensive traits
作者：Xiao, Bei;Jin, Yuli;Yu, Ningning;Wang, Jiaojiao;Qian, Zejun;Zhao, Ya;Ma, Pengtao;Qie, Yanmin;Sun, Nina;Liu, Wei;Yuan, Tangyu;Li, Linzhi;Wang, Xiaolu;Liu, Cheng;Wang, Fengtao
关键词：Wheat; Disease resistance; Stress tolerance; Agronomic and yield traits; Harmonious improvement
-
Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
作者：Mu, Yanjun;Yu, Ziyang;Xiao, Luning;Su, Fuyu;Zhang, Wenjing;Ma, Pengtao;Gong, Wenping;Guo, Jun;Han, Ran;Qie, Yanmin;Liu, Xueqing;Li, Linzhi;Sun, Nina;Liu, Wei;Wang, Jiangchun;Han, Guohao
关键词：wheat powdery mildew; pmYN99102; MAS; BSE-Seq; molecular mapping
-
Characterization of a new splicing variant of powdery mildew resistance gene Pm4 in synthetic hexaploid wheat YAV249
作者：Jin, Yuli;Gu, Tiantian;Liu, Hong;Han, Guohao;Shi, Zhipeng;Wang, Jing;An, Diaoguo;Zhao, He;Li, Xiuquan;Zhou, Yilin;Fan, Jieru;Liu, Wei;An, Diaoguo
关键词：Blumeria graminis f; sp; tritici; PmYAV; Pm4a; markers; marker-assisted selection
-
Wheat heat tolerance is impaired by heightened deletions in the distal end of 4AL chromosomal arm
作者：Zhai, Huijie;Yang, Shuling;Yan, Kunfang;Wu, Shuyu;Luo, Bingke;Du, Yi;Jin, Huaibing;Yin, Guihong;Zhang, Kunpu;Li, Guangwei;Wang, Daowen;Zhai, Huijie;Yang, Shuling;Yan, Kunfang;Wu, Shuyu;Luo, Bingke;Du, Yi;Jin, Huaibing;Yin, Guihong;Zhang, Kunpu;Li, Guangwei;Wang, Daowen;Zhai, Huijie;Jiang, Congcong;Zhao, Yue;Li, Yiwen;Jin, Huaibing;Liu, Xin;Lu, Fei;Zhang, Kunpu;Wang, Daowen;Zhai, Huijie;Ou, Xingqi;Zhang, Yanbin;Reynolds, Matthew;Qiao, Wenchen;Jiang, Zhikai;Peng, Tao;Gao, Derong;Hu, Wenjing;Wang, Jiangchun;Gao, Haitao
关键词：genetic mapping; haplotype analysis; heat stress tolerance; gene deletion; TaHST1; wheat
-
Genome-Wide Mapping of Quantitative Trait Loci Conferring All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat Landrace PI 181410
作者：Liu, Yan;Qie, Yanmin;Li, Xing;Wang, Meinan;Chen, Xianming;Qie, Yanmin;Li, Xing;Chen, Xianming
关键词：disease resistance; Puccinia striiformis; QTL mapping; stripe rust; Triticum aestivum; wheat
