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Uncovering seed vigor responsive miRNA in hybrid wheat and its parents by deep sequencing

文献类型： 外文期刊

作者： Yue, Jie-ru 1 ; Liu, Yong-jie 1 ; Yuan, Shao-hua 1 ; Sun, Hui 1 ; Lou, Hong-yao 1 ; Li, Yan-mei 1 ; Guo, Hao-yu 1 ; Liu, Zi-han 1 ; Zhang, Feng-ting 1 ; Zhai, Nuo 1 ; Zhang, Sheng-quan 1 ; Bai, Jian-fang 1 ; Zhang, Li-ping 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Hybrid Wheat, Beijing Key Lab Mol Genet Hybrid Wheat, Beijing 100097, Peoples R China

关键词： Two-line hybrid wheat; Seed vigor; MiRNA; RNA-seq

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundTwo-line hybrid wheat technology system is one way to harness wheat heterosis both domestically and internationally. Seed vigor is a crucial parameter for assessing seed quality, as enhanced seed vigor can lead to yield increments of over 20% to a certain extent. MicroRNAs (miRNAs) were known to participate in the development and vigor of seed in plants, but its impact on seed vigor in two-line hybrid wheat remains poorly elucidated.ResultsThe hybrid (BS1453/11GF5135) wheat exhibited superiority in seed vigor and anti-aging capacity, compared to its male parent (11GF5135, MP) and female parent (BS1453, FP). We identified four miRNAs associated with seed vigor, all of which are novel miRNAs. The majority of targets of miRNAs were related to ubiquitin ligases, kinases, sucrose synthases and hydrolases, involving in starch and sucrose metabolism, hydrolysis, catalysis, plant hormone signal transduction, and other pathways, which played crucial roles in seed development. Additionally, we also found miR531 was differentially expressed in both male parent and hybrid, and its target gene was a component of the E1 subunit of alpha-ketoate dehydrogenase complex, which interacted with dihydrolipoamide acetyltransferase (E2) and dihydrolipoyl dehydrogenase (E3). Finally, We established a presumptive interaction model to speculate the relationship of miR531 and seed vigor.ConclusionsThis study analyzed the seed vigor of two-line hybrid wheat, and screened seed vigor-related miRNAs. Meanwhile speculated the genetic relationship of hybrid and parents, in terms of miRNAs. Consequently, the present study provides new insights into the miRNA-mediated gene and protein interaction network that regulates seed vigor. These findings hold significance for enhancing the yield and quality of two-line hybrid wheat, facilitating its future applications.

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