文献类型： 外文期刊

作者： Wang, Jie ¹ ; Zhang, Li ² ; Wang, Shoudong ³ ; Wang, Xin ² ; Li, Suning ⁴ ; Gong, Pingping ⁵ ; Bai, Mengyan ² ; Paul, Arnav ⁶ ; Tvedt, Nathan ⁷ ; Ren, Hengrui ² ; Yang, Maoxiang ¹ ; Zhang, Zhihui ¹ ; Zhou, Shaodong ¹ ; Sun, Jiayi ³ ; Wu, Xianjin ¹ ; Kuang, Huaqin ² ; Du, Zhenghua ⁵ ; Dong, Yonghui ⁵ ; Shi, Xiaolei ⁸ ; Li, Meina ² ; Shukla, Diwakar ⁶ ; Yan, Long ⁸ ; Guan, Yuefeng ² ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Resources & Environm, Fuzhou 350002, Peoples R China

2.Guangzhou Univ, Innovat Ctr Mol Genet & Evolut, Sch Life Sci, Guangdong Prov Key Lab Plant Adaptat & Mol Design, Guangzhou 510006, Peoples R China

3.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, State Key Lab Black Soils Conservat & Utilizat, Changchun 130102, Peoples R China

4.Jiangxi Acad Agr Sci, Crop Inst, Jiangxi Prov Key Lab Oil Crops Genet Improvement, Nanchang 330200, Peoples R China

5.Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Sch Life Sci, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Peoples R China

6.Univ Illinois, Dept Chem, Urbana, IL 61801 USA

7.Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA

8.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Hebei Lab Crop Genet & Breeding, Shijiazhuang 050035, Peoples R China

9.Univ Illinois Champaign Urbana, Dept Chem & Biomol Engn, Urbana, IL 61801 USA

关键词： AlphaFold; CRISPR-Cas9; gene editing; oil content; precision breeding; soybean; SWEET transporter

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年 12 卷 23 期

页码：

收录情况： SCI

摘要： Enhancing the oil or protein content of soybean, a major crop for oil and protein production is highly desirable. GmSWEET10a encodes a sugar transporter that is strongly selected during domestication and breeding, increasing seed size and oil content. GmSWEET10b is functionally similar to GmSWEET10a, yet has not been artificially selected. Here, AlphaFold is used to find that C-terminal variants of GmSWEET10a can endow enhanced or reduced transport activity. Guided by AlphaFold, the functionality is improved for GmSWEET10a in terms of oil content through gene editing. Furthermore, novel GmSWEET10b haplotypes possessing strengthened or weakened sugar-transport capabilities that are absent in nature are engineered. Consequently, soybean oil content or protein content in independent GmSWEET10b gene-edited lines during multi-year and multi-site field trials is consistently increased, without negatively affecting yield. The study demonstrates that the combination of AlphaFold-guided protein design and gene editing has the potential to generate novel beneficial alleles, which can optimize protein function in the context of crop breeding.