文献类型： 外文期刊

作者： Zhong, Yang ¹ ; Xun, Weibing ⁴ ; Wang, Xiaohan ⁵ ; Tian, Shouwei ⁶ ; Zhang, Yancong ⁷ ; Li, Dawei ¹ ; Zhou, Yuan ⁸ ; Qin, Yuxuan ¹ ; Zhang, Bo ⁹ ; Zhao, Guangwei ¹⁰ ; Cheng, Xu ¹ ; Liu, Yaoguang ² ; Chen, Huiming ¹¹ ; Li, Legong ⁵ ; Osbourn, Anne ¹² ; Lucas, William J. ¹³ ; Huang, Sanwen ¹ ; Ma, Yongshuo ¹ ; Shang, Yi ⁹ ;

作者机构： 1.Chinese Acad Agr Sci, Shenzhen Branch, Guangdong Lab Lingnan Modern Agr, Agr Genom Inst Shenzhen,Genome Anal Lab,Minist Ag, Shenzhen, Peoples R China

2.South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Coll Life Sci, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Peoples R China

3.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, Sino Dutch Joint Lab Hort Genom,Inst Vegetables &, Beijing, Peoples R China

4.Nanjing Agr Univ, Jiangsu Prov Key Lab Solid Organ Waste Utilizat, Jiangsu Collaborat Innovat Ctr Solid Organ Wastes, Nanjing, Peoples R China

5.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China

6.Beijing Acad Agr & Forestry Sci, Natl Watermelon & Melon Improvement Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Key Lab Vegetable Germplasm Improvement, Beijing, Peoples R China

7.Harvard TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA USA

8.Huazhong Univ Sci & Technol, Sch Pharm, Tongji Med Coll, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan, Peoples R China

9.Yunnan Normal Univ, Yunnan Key Lab Potato Biol, CAAS YNNU YINMORE Joint Acad Potato Sci, Kunming, Yunnan, Peoples R China

10.Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou, Peoples R China

11.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha, Peoples R China

12.John Innes Ctr, Norwich Res Pk, Norwich, Norfolk, England

13.Univ Calif Davis, Coll Biol Sci, Dept Plant Biol, Davis, CA USA

14.MIT, Dept Chem Engn, Cambridge, MA 02139 USA

期刊名称：NATURE PLANTS （ 影响因子：17.352； 五年影响因子：19.328 ）

ISSN： 2055-026X

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收录情况： SCI

摘要： Root exudates play a key role in modulating the soil microbiota. The export of bitter triterpenes (mediated by a Multidrug and Toxic Compound Extrusion protein) shapes the rhizosphere, leading to robust disease resistance. Underground microbial ecosystems have profound impacts on plant health(1-5). Recently, essential roles have been shown for plant specialized metabolites in shaping the rhizosphere microbiome(6-9). However, the potential mechanisms underlying the root-to-soil delivery of these metabolites remain to be elucidated(10). Cucurbitacins, the characteristic bitter triterpenoids in cucurbit plants (such as melon and watermelon), are synthesized by operon-like gene clusters(11). Here we report two Multidrug and Toxic Compound Extrusion (MATE) proteins involved in the transport of their respective cucurbitacins, a process co-regulated with cucurbitacin biosynthesis. We further show that the transport of cucurbitacin B from the roots of melon into the soil modulates the rhizosphere microbiome by selectively enriching for two bacterial genera, Enterobacter and Bacillus, and we demonstrate that this, in turn, leads to robust resistance against the soil-borne wilt fungal pathogen, Fusarium oxysporum. Our study offers insights into how transporters for specialized metabolites manipulate the rhizosphere microbiota and thereby affect crop fitness.