文献类型： 外文期刊

作者： Huang, Wei ¹ ; Song, Yongkang ² ; Wang, Xiaonan ¹ ; Lin, Daiyan ⁴ ; Luo, Tuyan ² ; Cheng, Kaimin ⁵ ; Li, Qiaolian ⁶ ; Tian, Baoyu ¹ ; Zheng, Yongbiao ¹ ; He, Yongjin ¹ ;

作者机构： 1.Fujian Normal Univ, Coll Life Sci, Fuzhou, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Fuzhou, Fujian, Peoples R China

3.Fujian Acad Agr Sci, Fujian Key Lab Agroprod Qual & Safety, Fuzhou, Fujian, Peoples R China

4.Fujian Acad Agr Sci, Inst Food Sci & Technol, Fuzhou, Fujian, Peoples R China

5.Guangdong Yuehai Feed Grp Co LTD, Res Dept, Zhanjiang, Guangdong, Peoples R China

6.Fujian Prov Geriatr Hosp, Dept Gastroenterol, Fuzhou, Fujian, Peoples R China

关键词： Litopenaeus vannamei; docosahexaenoic acid; monoglycerides; lipidomics; intestinal microbiota

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：3.0； 五年影响因子：3.8 ）

ISSN：

年卷期： 2025 年 12 卷

页码：

收录情况： SCI

摘要： Docosahexaenoic acid (DHA) positively influences growth development, feed efficiency, physiological functions, and DHA accumulation in aquatic organisms. Recent mammalian studies demonstrate that DHA in the form of monoglycerides (MGs) is more conducive to facilitating the uptake and impact of DHA bioavailability than triglyceride (TG)-derived DHA. In this study, Schizochytrium-derived monoglyceride as a novel structured lipid form enriched in DHA-MGs was used to replace the fish oil of Litopenaeus vannamei diet and the effects of different DHA-MG levels on the growth performance, lipid composition of muscle and hepatopancreas, and intestinal microbiota of L. vannamei were investigated. The results showed that the diet with a high level of DHA-MGs distinctly increased the final weight, weight gain rate, and specific growth rate of L. vannamei. No notable variation in the biochemical makeup of shrimp muscle was detected among the four diets. The increase in DHA-MGs effectively facilitated the accumulation of DHA in the muscle and hepatopancreas of L. vannamei. In terms of lipidomics analysis, the addition of DHA-MGs significantly influenced the lipid species composition and DHA distribution in shrimp muscle and hepatopancreas. Additionally, the obtained results revealed that the addition of DHA-MGs improved the contents of diglycerides and triglycerides from the neutral lipid category with DHA and DHA located at specific positions on the glycerol backbone for shrimp muscle. Meanwhile, the lipid species from the glycerophospholipid category in shrimp tissues treated with four diets performed different variations. Besides that, the incorporation of DHA-MGs influenced the levels of microbial phylum and genus and the abundance of potential bacterial phenotypes for shrimp midgut. It was noted that the addition of DHA-MGs significantly decreased the abundance of potentially pathogenic bacteria, manifesting that DHA-MGs could readjust the intestinal microecological balance for intestinal health. In conclusion, the use of Schizochytrium-derived DHA-MGs to replace the fish oil in shrimp diet boosted the accumulation of DHA into tissues, modified the levels of lipid species with DHA and DHA located at specific positions on the glycerol backbone, and improved the intestinal health for the growth performance of L. vannamei.