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Molecular Characteristics and Functional Identification of a Key Alpha-Amylase-Encoding Gene AMY11 in Musa acuminata

文献类型： 外文期刊

作者： Sun, Peiguang 1 ; Zhu, Zhao 1 ; Jin, Zhiqiang 1 ; Xie, Jianghui 1 ; Miao, Hongxia 1 ; Liu, Juhua 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Sanya Res Inst, Natl Key Lab Trop Crop Biol Breeding, 4 Xueyuan Rd, Haikou 571101, Peoples R China

2.Hainan Univ, Coll Trop Crops, 58 Renmin Ave, Haikou 571100, Peoples R China

3.Chinese Acad Trop Agr Sci, Hainan Inst Trop Agr Resources, Hainan Key Lab Protect & Utilizat Trop Bioresource, Haikou 571101, Peoples R China

关键词： alpha-amylase; cis-acting elements; functional regulation; genome-wide identification; Musa acuminata L.; starch degradation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 14 期

页码：

收录情况： SCI

摘要： Alpha-amylase (AMY) plays a significant role in regulating the growth, development, and postharvest quality formation in plants. Nevertheless, little is known about the genome-wide features, expression patterns, subcellular localization, and functional regulation of AMY genes (MaAMYs) in the common starchy banana (Musa acuminata). Twelve MaAMY proteins from the banana genome database were clustered into two groups and contained a conserved catalytic domain. These MaAMYs formed collinear pairs with the AMYs of maize and rice. Three tandem gene pairs were found within the MaAMYs and are indicative of putative gene duplication events. Cis-acting elements of the MaAMY promoters were found to be involved in phytohormone, development, and stress responses. Furthermore, MaAMY02, 08, 09, and 11 were actively expressed during fruit development and ripening. Specifically, MaAMY11 showed the highest expression level at the middle and later stages of banana ripening. Subcellular localization showed that MaAMY02 and 11 were predominately found in the chloroplast, whereas MaAMY08 and 09 were primarily localized in the cytoplasm. Notably, transient attenuation of MaAMY11 expression resulted in an obvious increase in the starch content of banana fruit, while a significant decrease in starch content was confirmed through the transient overexpression of MaAMY11. Together, these results reveal new insights into the structure, evolution, and expression patterns of the MaAMY family, affirming the functional role of MaAMY11 in the starch degradation of banana fruit.

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