浙江省农业科学院机构知识库

Binding between elongation factor 1A and the 3MODIFIER LETTER PRIME-UTR of Chinese wheat mosaic virus is crucial for virus infection

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文献类型：外文期刊

作者： Chen, Xuan ¹ ; He, Long ² ; Xu, Miaoze ² ; Yang, Jin ¹ ; Li, Juan ² ; Zhang, Tianye ² ; Liao, Qiansheng ⁴ ; Zhang, Hengmu ³ ;

作者机构： 1.Northwest Agr & Forestry Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China

2.Ningbo Univ, State Key Lab Qual & Safety Agroprod, Inst Plant Virol, Ningbo, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou, Peoples R China

4.Zhejiang SCI Tech Univ, Coll Life Sci, Hangzhou, Peoples R China

关键词： 3MODIFIER LETTER PRIME-untranslated region; Chinese wheat mosaic virus; eukaryotic translation elongation factor eEF1A; stem-loop; tRNA-like structure; untranslated region

期刊名称：MOLECULAR PLANT PATHOLOGY （影响因子：5.663；五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2021 年 22 卷 11 期

页码：

收录情况： SCI

摘要： The Chinese wheat mosaic virus (CWMV) genome consists of two positive-strand RNAs that are required for CWMV replication and translation. The eukaryotic translation elongation factor (eEF1A) is crucial for the elongation of protein translation in eukaryotes. Here, we show that silencing eEF1A expression in Nicotiana benthamiana plants by performing virus-induced gene silencing can greatly reduce the accumulation of CWMV genomic RNAs, whereas overexpression of eEF1A in plants increases the accumulation of CWMV genomic RNAs. In vivo and in vitro assays showed that eEF1A does not interact with CWMV RNA-dependent RNA polymerase. Electrophoretic mobility shift assays revealed that eEF1A can specifically bind to the 3MODIFIER LETTER PRIME-untranslated region (UTR) of CWMV genomic RNAs. By performing mutational analyses, we determined that the conserved region in the 3MODIFIER LETTER PRIME-UTR of CWMV genomic RNAs is necessary for CWMV replication and translation, and that the sixth stem-loop (SL-6) in the 3MODIFIER LETTER PRIME-UTR of CWMV genomic RNAs plays a key role in CWMV infection. We conclude that eEF1A is an essential host factor for CWMV infection. This finding should help us to develop new strategies for managing CWMV infections in host plants.

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