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Gas Chromatography-Mass Spectrometry Metabolite Analysis Combined with Transcriptomics Reveals Genes Involved in Wax Biosynthesis in Allium fistulosum L.

文献类型： 外文期刊

作者： Xing, Jiayi 1 ; Xu, Huanhuan 1 ; Zhu, Mingzhao 1 ; Zhang, Yuchen 1 ; Bai, Mifeng 1 ; Zhou, Xuyang 1 ; Liu, Huiying 4 ; Wang, Yongqin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci BAAFS, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

3.Beijing Key Lab Vegetable Germplasms Improvement, Beijing 100097, Peoples R China

4.Shihezi Univ, Agr Coll, Dept Hort, Key Lab Special Fruits & Vegetables Cultivat Physi, Shihezi 832003, Peoples R China

5.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Peoples R China

关键词： Allium fistulosum L.; gas chromatography-mass spectrometry; transcriptomics; wax biosynthesis; mutant

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.6 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 11 期

页码：

收录情况： SCI

摘要： Cuticular waxes are essential for protecting plants from various environmental stresses. Allium fistulosum serves as an excellent model for investigating the regulatory mechanisms underlying cuticular wax synthesis with notable epidermal wax characteristics. A combination of gas chromatography-mass spectrometry (GC-MS) metabolite analysis and transcriptomics was used to investigate variations in metabolites and gene expression patterns between the wild type (WT) and glossy mutant type (gl2) of A. fistulosum. The WT surface had a large number of acicular and lamellar waxy crystals, whereas the leaf surface of gl2 was essentially devoid of waxy crystals. And the results revealed a significant decrease in the content of 16-hentriacontanone, the principal component of cuticular wax, in the gl2 mutant. Transcriptomic analysis revealed 3084 differentially expressed genes (DEGs) between WT and gl2. Moreover, we identified 12 genes related to fatty acid or wax synthesis. Among these, 10 DEGs were associated with positive regulation of wax synthesis, whereas 2 genes exhibited negative regulatory functions. Furthermore, two of these genes were identified as key regulators through weighted gene co-expression network analysis. Notably, the promoter region of AfisC5G01838 (AfCER1-LIKE1) exhibited a 258-bp insertion upstream of the coding region in gl2 and decreased the transcription of the AfCER1-LIKE1 gene. This study provided insights into the molecular mechanisms governing cuticular wax synthesis in A. fistulosum, laying the foundation for future breeding strategies.

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