文献类型： 外文期刊

作者： Li, Yang ¹ ; Ma, Xiao ¹ ; Xiao, Luo-Dan ² ; Yu, Ya-Nan ¹ ; Yan, Hui-Ling ³ ; Gong, Zhen-Hui ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Hort, Yangling 712100, Peoples R China

2.Yibin Res Inst Tea Ind, Yibin 644000, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Inst Cash Crops, Shijiazhuang 050051, Peoples R China

关键词： anthracnose; fungal pathogens; CaWRKY50; Colletotrichum scovillei; pepper

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 10 期

页码：

收录情况： SCI

摘要： Chili anthracnose is one of the most common and destructive fungal pathogens that affects the yield and quality of pepper. Although WRKY proteins play crucial roles in pepper resistance to a variety of pathogens, the mechanism of their resistance to anthracnose is still unknown. In this study, we found that CaWRKY50 expression was obviously induced by Colletotrichum scovillei infection and salicylic acid (SA) treatments. CaWRKY50-silencing enhanced pepper resistance to C. scovillei, while transient overexpression of CaWRKY50 in pepper increased susceptibility to C. scovillei. We further found that overexpression of CaWRKY50 in tomatoes significantly decreased resistance to C. scovillei by SA and reactive oxygen species (ROS) signaling pathways. Moreover, CaWRKY50 suppressed the expression of two SA-related genes, CaEDS1 (enhanced disease susceptibility 1) and CaSAMT1 (salicylate carboxymethyltransferase 1), by directly binding to the W-box motif in their promoters. Additionally, we demonstrated that CaWRKY50 interacts with CaWRKY42 and CaMIEL1 in the nucleus. Thus, our findings revealed that CaWRKY50 plays a negative role in pepper resistance to C. scovillei through the SA-mediated signaling pathway and the antioxidant defense system. These results provide a theoretical foundation for molecular breeding of pepper varieties resistant to anthracnose.