Co-suppression of the EIN2-homology gene LeEIN2 inhibits fruit ripening and reduces ethylene sensitivity in tomato
文献类型： 外文期刊
作者： Hu, Z. L. 1 ; Deng, L. 1 ; Chen, X. Q. 1 ; Wang, P. Q. 1 ; Chen, G. P. 1 ;
作者机构： 1.Chongqing Univ, Coll Bioengn, Chongqing 400030, Peoples R China
2.Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China
关键词： ethylene.;co-suppression;LeEIN2;Internet resource.
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）
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收录情况： SCI
摘要： EIN2 (ethylene insensitive 2) is a very important component in the ethylene signal transduction pathway. Recently, the genomic DNA and full-length cDNA of LeEIN2 (tomato EIN2) have been isolated in our laboratory. To reveal the function of LeEIN2, transgenic tomato plants with reduced expression levels of LeEIN2 were produced. The fruit ripening and expressions of ripening-related genes encoding polygalacturonase and TomLoxB were inhibited in the LeEIN2-silenced transgenic plants compared to the wild-type Ailsa Craig. In the seedling ethylene response assay, the transgenic tomato plants with reduced LeEIN2 expression exhibited ethylene insensitivity. These results indicate that LeEIN2 plays a critical role in regulating tomato fruit ripening and is a positive regulator in ethylene signal transduction pathway.
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